Skip Navigation


Plant and Cell Physiology Advance Access originally published online on December 20, 2007
Plant and Cell Physiology 2008 49(2):178-190; doi:10.1093/pcp/pcm175
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Supplementary Data
Right arrow All Versions of this Article:
49/2/178    most recent
pcm175v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (1)
Right arrowRequest Permissions
Google Scholar
Right arrow Articles by Mashiguchi, K.
Right arrow Articles by Suzuki, Y.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Mashiguchi, K.
Right arrow Articles by Suzuki, Y.
Agricola
Right arrow Articles by Mashiguchi, K.
Right arrow Articles by Suzuki, Y.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

© The Author 2007. Published by Oxford University Press on behalf of Japanese Society of Plant Physiologists. All rights reserved. For permissions, please email: journals.permissions@oxfordjournals.org

Defense-Related Signaling by Interaction of Arabinogalactan Proteins and β-Glucosyl Yariv Reagent Inhibits Gibberellin Signaling in Barley Aleurone Cells

Kiyoshi Mashiguchi1, Eriko Urakami1, Morifumi Hasegawa2, Kazutsuka Sanmiya1,4, Ichiro Matsumoto1, Isomaro Yamaguchi3, Tadao Asami1 and Yoshihito Suzuki1,*

1Department of Applied Biological Chemistry, University of Tokyo, Bunkyo-ku, Tokyo, 113-8657 Japan
2College of Agriculture, Ibaraki University, Ami, Ibaraki, 300-0393 Japan
3Department of Biotechnology, Maebashi Institute of Technology, Maebashi, Gunma, 371-0816 Japan

*Corresponding author: E-mail, ayoshi{at}pgr1.ch.a.u-tokyo.ac.jp; Fax, +81-3-5841-8025.


  Abstract

Arabinogalactan proteins (AGPs) are hydroxyproline-rich glycoproteins present at the plasma membrane and in extracellular spaces. A synthetic chemical, β-glucosyl Yariv reagent (β-GlcY), binds specifically to AGPs. We previously reported that gibberellin signaling is specifically inhibited by β-GlcY treatment in barley aleurone protoplasts. In the present study, we found that β-GlcY also inhibited gibberellin-induced programmed cell death (PCD) in aleurone cells. We examined the universality and specificity of the inhibitory effect of β-GlcY on gibberellin signaling using microarray analysis and found that β-GlcY was largely effective in repressing gibberellin-induced gene expression. In addition, >100 genes were up-regulated by β-GlcY in a gibberellin-independent manner, and many of these were categorized as defense-related genes. Defense signaling triggered by several defense system inducers such as jasmonic acid and a chitin elicitor could inhibit gibberellin-inducible events such as {alpha}-amylase secretion, PCD and expression of some gibberellin-inducible genes in aleurone cells. Furthermore, β-GlcY repressed the gibberellin-inducible Ca2+-ATPase gene which is important for gibberellin-dependent gene expression, and induced known repressors of gibberellin signaling, two WRKY genes and a NAK kinase gene. These effects of β-GlcY were also phenocopied by the chitin elicitor and/or jasmonic acid. These results indicate that gibberellin signaling is under the regulation of defense-related signaling in aleurone cells. It is also probable that AGPs are involved in the perception of stimuli causing defense responses.

Keywords: Aleurone layer — Arabinogalactan protein — Barley — Defense response — Gibberellin — Protoplast

Abbreviations: AGP, arabinogalactan protein; AOS, allene oxide synthase; CH, N-acetylchitohexaose; DMSO, dimethylsulfoxide; FDA, fluorescein diacetate; FDR, false discovery rate; {alpha}-GalY, {alpha}-galactosyl Yariv reagent; GPI, glycosylphosphatidylinositol; β-GlcY, β-glucosyl Yariv reagent; JA, jasmonic acid; LOX, lipoxygenase; OPDA, 12-oxophytodienoic acid; PCD, programmed cell death; RT–PCR, reverse transcription–PCR; SA, salicylic acid.

4Present address: Department of Bioresources Engineering, Okinawa National College of Technology, Nago, Okinawa, 905-2192 Japan.

(Received September 19, 2007; Accepted December 17, 2007)
Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?




Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.