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Plant and Cell Physiology Advance Access originally published online on October 30, 2008
Plant and Cell Physiology 2008 49(12):1911-1916; doi:10.1093/pcp/pcn161
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© The Author 2008. Published by Oxford University Press on behalf of Japanese Society of Plant Physiologists. All rights reserved. For permissions, please email: journals.permissions@oxfordjournals.org

Short Communication

Molecular Identity of Uncoupling Proteins in Thermogenic Skunk Cabbage

Yasuko Ito-Inaba1,*, Yamato Hida1, Hitoshi Mori2 and Takehito Inaba3

1Cryobiofrontier Research Center, Faculty of Agriculture, Iwate University, Morioka, 020-8550 Japan
2Graduate School of Bio-agricultural Sciences, Nagoya University, Furo-cho, Chikusa, Nagoya, 464-8601 Japan
3The 21st Century Centers of Excellence Program, Cryobiofrontier Research Center, Iwate University, Morioka, 020-8550 Japan

*Corresponding author: E-mail, ykoito{at}iwate-u.ac.jp; Fax, +81-19-621-6200


  Abstract

Thermogenic skunk cabbage has been reported to have two types of uncoupling protein (UCP), a typical 6-transmembrane (TM) SrUCPA and an atypical 5-TM SrUCPB. To verify further the role of SrUCPs in thermogenic skunk cabbage, we examined the molecular identity of SrUCPs in more detail. Both mRNA and genomic analyses supported the presence of SrUCPA, but not SrUCPB. Furthermore, SrUCP protein purified from spadix mitochondria was identified as SrUCPA by mass spectrometry. These results clearly indicate that SrUCPA is the major expressed UCP in skunk cabbage, and the presence of atypical SrUCPB is unlikely to be associated with thermogenesis of skunk cabbage.

Keywords: mRNA splicing - Plant mitochondria - Reproductive organ - Skunk cabbage - Thermogenesis - Uncoupling protein

Abbreviations: AOX, alternative oxidase; MALDI, matrix-assisted laser desorption ionization; MS, mass spectrometry; RT–PCR, reverse transcription–PCR; TEV, tobacco etch virus; TM, transmembrane; UCP, uncoupling protein; UTR, untranslated region

(Received October 1, 2008; Accepted October 23, 2008)
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