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Plant and Cell Physiology Advance Access originally published online on October 14, 2008
Plant and Cell Physiology 2008 49(12):1898-1901; doi:10.1093/pcp/pcn151
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© The Author 2008. Published by Oxford University Press on behalf of Japanese Society of Plant Physiologists. All rights reserved. For permissions, please email: journals.permissions@oxfordjournals.org

Short Communication

Abscisic Acid Does Not Disrupt Either the Arabidopsis FCA–FY Interaction or its Rice Counterpart in vitro

Yun Hee Jang, Jeong Hwan Lee and Jeong-Kook Kim*

Plant Signaling Network Research Center, School of Life Sciences and Biotechnology, Korea University, Seoul, 136-701, Korea

*Corresponding author: E-mail, jkkim{at}korea.ac.kr; Fax, +82-2-927-9028.


  Abstract

We examined the effect of (+)-ABA on the in vitro interaction of rice FCA and FY homologs, OsFCA and OsFY. From this analysis, we found no disruption of the OsFCA–OsFY complexes by ABA treatment. This result prompted us to examine the effect of ABA on the FCA–FY interaction. In these experiments, we could not reproduce the inhibitory effect of (+)-ABA on the interaction between FCA and FY. Based on these combined results, we believe that the inhibitory effect of (+)-ABA on the FCA–FY interaction should be cautiously reconsidered.

Keywords: ABA — ABA receptor — FCA and FY interaction — GST pull-down assay — OsFCA and OsFY interaction

Abbreviations: CHLH, Mg-chelatase H subunit; GCR2, G protein-coupled receptor 2; GST, glutathione S-transferase; RRM, RNA recognition motif

(Received September 12, 2008; Accepted October 7, 2008)
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