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Plant and Cell Physiology Advance Access originally published online on October 9, 2008
Plant and Cell Physiology 2008 49(12):1783-1791; doi:10.1093/pcp/pcn152
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© The Author 2008. Published by Oxford University Press on behalf of Japanese Society of Plant Physiologists. All rights reserved. For permissions, please email: journals.permissions@oxfordjournals.org

Interplay of RNA Pol IV and ROS1 During Post-Embryonic 5S rDNA Chromatin Remodeling

Julien Douet1, Bertrand Blanchard1,2, Claudine Cuvillier1 and Sylvette Tourmente1,*

1CNRS, UMR 6247 GReD, Clermont Université, INSERM, 24 Avenue des Landais, 63177 Aubière Cedex, France

*Corresponding author: E-mail, sylvette.tourmente{at}univ-bpclermont.fr; Fax, +33-4-73-40-77-77.


  Abstract

We have investigated the chromatin structure of 5S rDNA, a heterochromatic pericentromeric tandemly repeated family, at 2, 3, 4 and 5 days post-germination. Our results revealed a large-scale reorganization of 5S rDNA chromatin that occurs during the first days of development. Unexpectedly, there is a decondensation followed by a ‘re’condensation of 5S rDNA chromatin, to obtain almost mature nuclei 5 d post-germination. The reorganization of 5S rDNA chromatin is accompanied by a rapid and active demethylation of 5S rDNA mediated by the ROS1 (repressor of silencing 1) demethylase, whereas the plant-specific RNA polymerase IV (Pol IV) is essential to the 5S chromatin ‘re’condensation. In conclusion, Pol IV and ROS1 collaborate to unlock the 5S rDNA chromatin inherited from the seed, and establish adult features.

Keywords: Arabidopsis thaliana - Chromatin - RNA polymerase IV - ROS1 - 5S rDNA

Abbreviations: BAC, bacterial artificial chromosome; CC, chromocenter; DAPI, 4',6-diamidino-2-phenylindole; FISH, fluorescence in situ hybridization; NOR, nucleolar organizing region; Pol IV, RNA polymerase IV; RNAi, RNA interference; ROS1, repressor of silencing 1; siRNA, small interfering RNA; TSI, transcriptionally silent information; WT, wild type

2Present address: CERMAV-CNRS, Université Joseph Fourier, 601 rue de la Chimie, BP53 38041 Grenoble Cedex 9, France.

(Received September 13, 2008; Accepted October 7, 2008)
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