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Plant and Cell Physiology Advance Access originally published online on September 10, 2008
Plant and Cell Physiology 2008 49(11):1672-1677; doi:10.1093/pcp/pcn139
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© The Author 2008. Published by Oxford University Press on behalf of Japanese Society of Plant Physiologists. All rights reserved. For permissions, please email: journals.permissions@oxfordjournals.org

Properties of Mutants of Synechocystis sp. Strain PCC 6803 Lacking Inorganic Carbon Sequestration Systems

Min Xu1, Gábor Bernát2, Abhay Singh3, Hualing Mi1, Matthias Rögner2, Himadri B. Pakrasi3 and Teruo Ogawa1,2,3,*

1 National Laboratory of Plant Molecular Genetics, Institute of Plant Physiology and Ecology, Shanghai Institute for Biological Sciences, Graduate courses of the Chinese Academy of Sciences, Shanghai, 200032, PR China
2 Plant Biochemistry, Ruhr-University Bochum, D-44801 Bochum, Germany
3 Department of Biology, Box 1137, Washington University, St Louis, MO 63130, USA

*Corresponding author: E-mail, ogawater{at}xd6.so-net.ne.jp


  Abstract

A mutant ({Delta}5) of Synechocystis sp. strain PCC 6803 constructed by inactivating five inorganic carbon sequestration systems did not take up CO2 or HCO3– and was unable to grow in air with or without glucose. The {Delta}4 mutant in which BicA is the only active inorganic carbon sequestration system showed low activity of HCO3– uptake and grew under these conditions but more slowly than the wild-type strain. The {Delta}5 mutant required 1.7% CO2 to attain half the maximal growth rate. Electron transport activity of the mutants was strongly inhibited under high light intensities, with the {Delta}5 mutant more susceptible to high light than the {Delta}4 mutant. The results implicated the significance of carbon sequestration in dissipating excess light energy.

Keywords: CO2 and HCO3– uptake - CO2-concentrating mechanism (CCM) - Cyanobacteria - Electron transport - Inorganic carbon sequestration

Abbreviations: CCM, CO2-concentrating mechanism; Ci, inorganic carbon; (r)ETR, (relative) electron transport rate; H, high CO2-grown; L, low CO2-induced; PAR, photosynthetically active radiation; WT, wild type.

(Received June 25, 2008; Accepted September 8, 2008)
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