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Plant and Cell Physiology Advance Access originally published online on August 20, 2008
Plant and Cell Physiology 2008 49(10):1547-1562; doi:10.1093/pcp/pcn119
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© The Author 2008. Published by Oxford University Press on behalf of Japanese Society of Plant Physiologists. All rights reserved. For permissions, please email: journals.permissions@oxfordjournals.org

Arabidopsis thaliana Has a Set of J Proteins in the Endoplasmic Reticulum that are Conserved from Yeast to Animals and Plants

Masaya Yamamoto1, Daisuke Maruyama1, Toshiya Endo1,2,3 and Shuh-ichi Nishikawa1,*

1 Department of Chemistry, Graduate School of Science, Nagoya University, Chikusa-ku, Nagoya, 464-8602 Japan
2 The Institute for Advanced Research, Nagoya University, Chikusa-ku, Nagoya, 464-8602 Japan
3 The Core Research for Evolutional Science and Technology (CREST), Japan Science and Technology Corporation (JST), Nagoya University, Chikusa-ku, Nagoya, 464-8602 Japan

*Corresponding author: E-mail, shuh{at}biochem.chem.nagoya-u.ac.jp; Fax, +81-52-78-2947.


  Abstract

J domain-containing proteins (J proteins) are functional partners for heat shock protein 70 (Hsp70) molecular chaperones and mediate various cellular processes by regulating activities of Hsp70. Budding yeast has three J proteins in the endoplasmic reticulum (ER): Scj1p and Jem1p functioning in protein folding and quality control in the ER, and Sec63p functioning in protein translocation across the ER membrane as partners for BiP, an Hsp70 in the ER. Here we report that Arabidopsis thaliana has orthologs of these yeast ER J proteins, which we designated as AtERdj3A, AtERdj3B, AtP58IPK, AtERdj2A and AtERdj2B. Tunicamycin treatment of Arabidopsis cells, which causes ER stress, led to up-regulation of AtERdj3A, AtERdj3B, AtP58IPK and AtERdj2B. Subcellular fractionation analyses showed their ER localization, indicating that the identified J proteins indeed function as partners for BiP in Arabidopsis cells. Since expression of AtERdj3A, AtERdj3B and AtP58IPK partially suppressed the growth defects of the yeast jem1{Delta}scj1{Delta} mutant, they have functions similar to those of Scj1p and Jem1p. T-DNA insertions of the AtERDJ2A gene resulted in pollen germination defects, probably reflecting its essential function in protein translocation. These results suggest that A. thaliana has a set of ER J proteins structurally and functionally conserved from yeast to plants.

Keywords: Arabidopsis thaliana - Endoplasmic reticulum - J proteins - Molecular chaperone - Quality control

Abbreviations: CaMV, cauliflower mosaic virus; DAPI, 4',6-diamidino-2-phenylindole; ER, endoplasmic reticulum; GFP, green fluorescent protein; Hsp, heat shock protein; mRFP, monomeric red fluorescent protein; ORF, open reading frame; PERK, PKR-like endoplasmic reticulum kinase; PMSF, phenylmethylsulfonyl fluoride; TPR, tetratricopeptide repeat; UPR, unfolded protein response.

(Received May 23, 2008; Accepted August 16, 2008)
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