Skip Navigation


Plant and Cell Physiology Advance Access originally published online on September 6, 2008
Plant and Cell Physiology 2008 49(10):1417-1428; doi:10.1093/pcp/pcn128
This Article
Right arrow Full Text Freely available
Right arrow FREE Full Text (PDF) Freely available
Right arrow Supplementary Data
Right arrowOA All Versions of this Article:
49/10/1417    most recent
pcn128v2
pcn128v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Related articles in PCP
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (4)
Google Scholar
Right arrow Articles by Hobo, T.
Right arrow Articles by Matsuoka, M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Hobo, T.
Right arrow Articles by Matsuoka, M.
Agricola
Right arrow Articles by Hobo, T.
Right arrow Articles by Matsuoka, M.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

© The Author 2008. Published by Oxford University Press on behalf of Japanese Society of Plant Physiologists. All rights reserved.
The online version of this article has been published under an open access model. Users are entitled to use, reproduce, disseminate, or display the open access version of this article for non-commercial purposes provided that: the original authorship is properly and fully attributed; the Journal and the Japanese Society of Plant Physiologists are attributed as the original place of publication with the correct citation details given; if an article is subsequently reproduced or disseminated not in its entirety but only in part or as a derivative work this must be clearly indicated. For commercial re-use, please contact journals.permissions@oxfordjournals.org

Special Issue - Regular Paper

Various Spatiotemporal Expression Profiles of Anther-Expressed Genes in Rice

Tokunori Hobo1,11, Keita Suwabe2,11, Koichiro Aya1, Go Suzuki3, Kentaro Yano4, Takeshi Ishimizu5, Masahiro Fujita6, Shunsuke Kikuchi4, Kazuki Hamada4, Masumi Miyano2,7, Tomoaki Fujioka2, Fumi Kaneko2, Tomohiko Kazama2,7, Yoko Mizuta6, Hirokazu Takahashi8, Katsuhiro Shiono8, Mikio Nakazono8, Nobuhiro Tsutsumi8, Yoshiaki Nagamura9, Nori Kurata6, Masao Watanabe2,7,* and Makoto Matsuoka1,*

1Bioscience and Biotechnology Center, Nagoya University, Furocho, Chikusa, Nagoya, 464-8601 Japan
2Graduate School of Life Sciences, Tohoku University, Sendai, 980-8577 Japan
3Division of Natural Science, Osaka Kyoiku University, Kashiwara, 582-8582 Japan
4Faculty of Agriculture, Meiji University, Kawasaki, 214-8571 Japan
5Department of Chemistry, Graduate School of Science, Osaka University, Osaka, 560-0043 Japan
6Plant Genetics Laboratory, National Institute of Genetics, Mishima, 411-8540 Japan
7The 21st Century Center of Excellence Program, Iwate University, Morioka, 020-8550 Japan
8Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo, 113-8657 Japan
9Division of Genome and Biodiversity Research, National Institute of Agrobiological Sciences, Tsukuba, 305-8602 Japan
10Faculty of Science, Tohoku University, Sendai, 980-8587 Japan

*Corresponding authors: Masao Watanabe, E-mail, nabe{at}ige.tohoku.ac.jp; Fax, +81-22-217-5683; Makoto Matsuoka, E-mail, makoto{at}nuagr1.agr.nagoya-u.ac.jp; Fax, +81-52-789-5226


  Abstract

The male gametophyte and tapetum play different roles during anther development although they are differentiated from the same cell lineage, the L2 layer. Until now, it has not been possible to delineate their transcriptomes due to technical difficulties in separating the two cell types. In the present study, we characterized the separated transcriptomes of the rice microspore/pollen and tapetum using laser microdissection (LM)-mediated microarray. Spatiotemporal expression patterns of 28,141 anther-expressed genes were classified into 20 clusters, which contained 3,468 (12.3%) anther-enriched genes. In some clusters, synchronous gene expression in the microspore and tapetum at the same developmental stage was observed as a novel characteristic of the anther transcriptome. Noteworthy expression patterns are discussed in connection with gene ontology (GO) categories and gene annotations, which are related to important biological events in anther development, such as pollen maturation, pollen germination, pollen tube elongation and pollen wall formation.

Keywords: Anther - Gene ontology - Laser microdissection - Microarray - Oryza sativa L - Synchronous gene expression

Abbreviations: BC, bicellular; GO, gene ontology; LM, laser microdissection; MEI, meiosis; miRNA, microRNA; TC, tri­cellular; TET, tetrad; UN, uninuclear; VSN, variance stabiliza­tion normalization

11These authors contributed equally to this work.

(Received July 20, 2008; Accepted August 30, 2008)
Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?

Related articles in PCP:

Towards a Comprehensive Understanding of Molecular Mechanisms of Sexual Reproduction in Higher Plants
Masao Watanabe
PCP 2008 49: 1404-1406. [Extract] [FREE Full Text]  



This article has been cited by other articles:


Home page
 Plant Cell PhysiolHome page
M. Endo, T. Tsuchiya, K. Hamada, S. Kawamura, K. Yano, M. Ohshima, A. Higashitani, M. Watanabe, and M. Kawagishi-Kobayashi
High Temperatures Cause Male Sterility in Rice Plants with Transcriptional Alterations During Pollen Development
Plant Cell Physiol., November 1, 2009; 50(11): 1911 - 1922.
[Abstract] [Full Text] [PDF]

Home page
 Plant Cell PhysiolHome page
G. Suzuki
Recent Progress in Plant Reproduction Research: The Story of the Male Gametophyte through to Successful Fertilization
Plant Cell Physiol., November 1, 2009; 50(11): 1857 - 1864.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
M. Mihara, T. Itoh, and T. Izawa
SALAD database: a motif-based database of protein annotations for plant comparative genomics
Nucleic Acids Res., October 23, 2009; (2009) gkp831v1.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
Compiled by, F. Tooke, T. Chiurugwi, and N. Battey
Flowering Newsletter bibliography for 2008
J. Exp. Bot., June 23, 2009; (2009) erp154v1.
[Full Text] [PDF]

Home page
 J Exp BotHome page
Z. A. Wilson and D.-B. Zhang
From Arabidopsis to rice: pathways in pollen development
J. Exp. Bot., April 1, 2009; 60(5): 1479 - 1492.
[Abstract] [Full Text] [PDF]

Home page
 Plant Cell PhysiolHome page
K. Kurasawa, A. Matsui, R. Yokoyama, T. Kuriyama, T. Yoshizumi, M. Matsui, K. Suwabe, M. Watanabe, and K. Nishitani
The AtXTH28 Gene, a Xyloglucan Endotransglucosylase/Hydrolase, is Involved in Automatic Self-Pollination in Arabidopsis thaliana
Plant Cell Physiol., February 1, 2009; 50(2): 413 - 422.
[Abstract] [Full Text] [PDF]

Home page
 Plant Cell PhysiolHome page
K. Suwabe, G. Suzuki, H. Takahashi, K. Shiono, M. Endo, K. Yano, M. Fujita, H. Masuko, H. Saito, T. Fujioka, et al.
Separated Transcriptomes of Male Gametophyte and Tapetum in Rice: Validity of a Laser Microdissection (LM) Microarray
Plant Cell Physiol., October 1, 2008; 49(10): 1407 - 1416.
[Abstract] [Full Text] [PDF]

Home page
 Plant Cell PhysiolHome page
M. Mihara, T. Itoh, and T. Izawa
In Silico Identification of Short Nucleotide Sequences Associated with Gene Expression of Pollen Development in Rice
Plant Cell Physiol., October 1, 2008; 49(10): 1451 - 1464.
[Abstract] [Full Text] [PDF]

Home page
 Plant Cell PhysiolHome page
M. Watanabe
Towards a Comprehensive Understanding of Molecular Mechanisms of Sexual Reproduction in Higher Plants
Plant Cell Physiol., October 1, 2008; 49(10): 1404 - 1406.
[Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.