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Plant and Cell Physiology Advance Access originally published online on November 14, 2007
Plant and Cell Physiology 2008 49(1):11-18; doi:10.1093/pcp/pcm158
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© The Author 2007. Published by Oxford University Press on behalf of Japanese Society of Plant Physiologists. All rights reserved. For permissions, please email: journals.permissions@oxfordjournals.org

Binary Reducing Equivalent Pathways Using NADPH-Thioredoxin Reductase and Ferredoxin-Thioredoxin Reductase in the Cyanobacterium Synechocystis sp. Strain PCC 6803

Shoko Hishiya1,4, Wakako Hatakeyama1,4, Yoko Mizota1,4, Naomi Hosoya-Matsuda1,2, Ken Motohashi1, Masahiko Ikeuchi3 and Toru Hisabori1,*

1Chemical Resources Laboratory, Tokyo Institute of Technology, Nagatsuta 4259-R1-8, Midori-Ku, Yokohama, 226-8503 Japan
2Department of Biological Sciences, Kanagawa University, Hiratsuka, Kanagawa, 259-1293 Japan
3Department of Life Science (Biology), University of Tokyo, Komaba 3-8-1, Meguro-Ku, Tokyo, 153-8902 Japan

*Corresponding author: E-mail, thisabor{at}res.titech.ac.jp; Fax, +81-45-924-5277.


  Abstract

Thioredoxin (Trx) is a small ubiquitous protein involved in the disulfide–dithiol exchange reaction occurring in cells and organelles. In vivo, Trx is reduced by Trx reductase using NADPH or photosynthetically produced reducing equivalents, and the reduced form Trx takes on the physiological functions. In the cyanobacterium Synechocystis sp. PCC6803, two Trx reductases, ferredoxin-Trx reductase (FTR) and NADPH-Trx reductase (NTR), and four typical Trx isoforms have been identified by genomic analysis. Based on analysis of the physiological features of the Trx reductase disruptants, we found that the NTR–Trx pathway is important for the antioxidant system, whereas the FTR–Trx pathway may play a more important role in the control of cell growth rate. In addition, by quantification of Trx abundance in the wild-type and the disruptant Synechocystis cells, we found that the gene product of slr0623, the homolog of m-type Trx in higher plants, is the most abundant Trx, and that accumulation of Trx isoforms occurs dependent on the expression of the other redox-related proteins. A study of the binary reducing equivalent pathways in cyanobacterial cells is reported here.

Keywords: Cyanobacteria - Ferredoxin thioredoxin reductase - NADPH thioredoxin reductase - Reducing equivalent - Thioredoxin

Abbreviations: AMS, 4-acetamido-4'-maleimidyl-stilbene-2,2'-disulfonate; DTT, dithiothreitol; FTR, ferredoxin thioredoxin reductase; GT, glucose-tolerant; MV, methylviologen; NTR, NADPH thioredoxin reductase; ROS, reactive oxygen species; Trx, thioredoxin; WT, wild type.

4These authors contributed equally to this work.

(Received October 3, 2007; Accepted November 8, 2007)
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