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Plant and Cell Physiology Advance Access originally published online on July 29, 2007
Plant and Cell Physiology 2007 48(9):1374-1378; doi:10.1093/pcp/pcm097
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© The Author 2007. Published by Oxford University Press on behalf of Japanese Society of Plant Physiologists. All rights reserved. For permissions, please email: journals.permissions@oxfordjournals.org

Short Communication

Translation of psbC mRNAs Starts from the Downstream GUG, not the Upstream AUG, and Requires the Extended Shine–Dalgarno Sequence in Tobacco Chloroplasts

Hiroshi Kuroda1, Haruka Suzuki1, Takahiro Kusumegi2,4, Tetsuro Hirose2,5, Yasushi Yukawa1 and Masahiro Sugiura1,2,3,*

1Graduate School of Natural Sciences, Nagoya City University, Yamanohata, Mizuho, Nagoya, 467-8501 Japan
2Center for Gene Research, Nagoya University, Nagoya, 464-8602 Japan
3Sugiyama Human Research Center, Sugiyama Jogakuen University, Nagoya, 464-8662 Japan

*Corresponding author: E-mail, sugiura{at}nsc.nagoya-cu.ac.jp; Fax, +81-52-781-7196.


  Abstract

The plastid gene psbC encodes the CP43 subunit of PSII. Most psbC mRNAs of many organisms possess two possible initiation codons, AUG and GUG, and their coding regions are generally annotated from the upstream AUG. Using a chloroplast in vitro translation system, we show here that translation of the tobacco plastid psbC mRNA initiates from the GUG. This mRNA possesses a long Shine–Dalgarno (SD)-like sequence, GAGGAGGU, nine nucleotides upstream of the GUG. Point mutations in this sequence abolished translation, suggesting that a strong interaction between this extended SD-like sequence and the 3' end of 16S rRNA facilitates translation initiation from the GUG.

Keywords: GUG codon - In vitro - psbC - Shine–Dalgarno sequence - Tobacco - Translation

Abbreviations: mGFP, modified green fluorescent protein; nt, nucleotides; SD, Shine–Dalgarno; UTR, untranslated region.

4Present address: Toyota Motor Corporation, Toyota, Toyota, 471-8572 Japan.

5Present address: National Institute of Advanced Industrial Science and Technology, Tokyo, 135-0064 Japan.

(Received May 16, 2007; Accepted July 21, 2007)
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