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Plant and Cell Physiology Advance Access originally published online on July 10, 2007
Plant and Cell Physiology 2007 48(8):1207-1218; doi:10.1093/pcp/pcm090
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© The Author 2007. Published by Oxford University Press on behalf of Japanese Society of Plant Physiologists. All rights reserved. For permissions, please email: journals.permissions@oxfordjournals.org

The Jasmonate-Induced Expression of the Nicotiana tabacum Leaf Lectin

Nausicaä Lannoo1, Gianni Vandenborre1,2, Otto Miersch3, Guy Smagghe2, Claus Wasternack3, Willy J. Peumans1 and Els J. M. Van Damme1,*

1Laboratory of Biochemistry and Glycobiology, Department of Molecular Biotechnology, Ghent University, Coupure Links 653, B-9000 Gent, Belgium
2Laboratory of Agrozoology, Department of Crop Protection, Ghent University, Coupure Links 653, B-9000 Gent, Belgium
3Department of Natural Product Biotechnology, Leibniz Institute of Plant Biochemistry, Weinberg 3, D-06120 Halle/Saale, Germany

*Corresponding author: E-mail, ElsJM.VanDamme{at}UGent.be; Fax, +32-92646219.


  Abstract

Previous experiments with tobacco (Nicotiana tabacum L. cv Samsun NN) plants revealed that jasmonic acid methyl ester (JAME) induces the expression of a cytoplasmic/nuclear lectin in leaf cells and provided the first evidence that jasmonates affect the expression of carbohydrate-binding proteins in plant cells. To corroborate the induced accumulation of relatively large amounts of a cytoplasmic/nuclear lectin, a detailed study was performed on the induction of the lectin in both intact tobacco plants and excised leaves. Experiments with different stress factors demonstrated that the lectin is exclusively induced by exogeneously applied jasmonic acid and JAME, and to a lesser extent by insect herbivory. The lectin concentration depends on leaf age and the position of the tissue in the leaf. JAME acts systemically in intact plants but very locally in excised leaves. Kinetic analyses indicated that the lectin is synthesized within 12 h exposure time to JAME, reaching a maximum after 60 h. After removal of JAME, the lectin progressively disappears from the leaf tissue. The JAME-induced accumulation of an abundant nuclear/cytoplasmic lectin is discussed in view of the possible role of this lectin in the plant.

Keywords: Inducible protein - Jasmonate - Lectin - Nicotiana tabacum - Spodoptera littoralis - Tobacco

Abbreviations: AOC, allene oxide cyclase; BA, 6-benzylaminopurine; DMSO, dimethylsulfoxide; GA3; GA3, gibberellic acid; JA, jasmonic acid; JAME, jasmonic acid methyl ester; 12-OH-JA, tuberonic acid; Nictaba, Nicotiana tabacum agglutinin; OPDA, 12-oxo-phytodienoic acid; RT–PCR, reverse transcription–PCR; SA, salicylic acid

(Received June 2, 2007; Revision received July 7, 2007. )
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