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Plant and Cell Physiology Advance Access originally published online on May 3, 2007
Plant and Cell Physiology 2007 48(6):881-885; doi:10.1093/pcp/pcm052
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© The Author 2007. Published by Oxford University Press on behalf of Japanese Society of Plant Physiologists. All rights reserved. For permissions, please email: journals.permissions@oxfordjournals.org

Short Communication

Multiple Intracellular Locations of Lon Protease in Arabidopsis: Evidence for the Localization of AtLon4 to Chloroplasts

Oren Ostersetzer1,*, Yusuke Kato2, Zach Adam3 and Wataru Sakamoto2

1Agricultural Research Organization, Volcani Center, Bet Dagan 50250, Israel
2Research Institute for Bioresources, Okayama University, Kurashiki, Okayama, 710-0046 Japan
3The Robert H. Smith Institute of Plant Sciences and Genetics in Agriculture, The Hebrew University of Jerusalem, Rehovot 76100, Israel

*Corresponding author: E-mail, biranos{at}volcani.agri.gov.il; Fax, +972-3-966-9583.


  Abstract

Arabidopsis contains four Lon protease-like proteins (AtLon1–AtLon4), predicted to be localized in different cellular organelles, including mitochondria, peroxisomes and plastids. A notable question is whether Lon is present in chloroplasts, since it is absent from cyanobacteria and thus appears to have been lost during the evolution of photosynthetic organisms. Based on in vivo transient assays, we found that AtLon4 is dually targeted to both mitochondria and chloroplasts. Furthermore, immunoblot analysis localized AtLon4 to the thylakoids. Thus, in spite of its absence from basal photosynthetic organisms, our results suggest the presence of Lon in plant plastids.

Keywords: AAA family - ATP-dependent protease - Chloroplasts - Lon - Mitochondria - Peroxisomes

Abbreviations: AAA, ATPases associated with various cellular activities; CF1, coupling factor-1 of the chloroplast ATP synthase complex; EST, expressed sequence tag; GFP, green fluorescent protein; IPTG, isopropyl-ß-D-thiogalactophyranoside; PsbO, 33 kDa subunit of oxygen evolving complex of PSII; RT–PCR, reverse transcription–PCR; Rubisco, ribulose-1,5-bisphosphate carboxylase/oxygenase; SHMT, serine-hydroxymethyltransferase

(Received March 11, 2007; Accepted April 24, 2007)
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