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Plant and Cell Physiology Advance Access originally published online on April 22, 2007
Plant and Cell Physiology 2007 48(6):875-880; doi:10.1093/pcp/pcm047
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© The Author 2007. Published by Oxford University Press on behalf of Japanese Society of Plant Physiologists. All rights reserved. For permissions, please email: journals.permissions@oxfordjournals.org

Short Communication

Regulatory Properties of Potato–Arabidopsis Hybrid ADP-Glucose Pyrophosphorylase

Tiziana Ventriglia1, Miguel A. Ballicora2,3, Pedro Crevillén1,4, Jack Preiss2 and José M. Romero1,*

1Instituto de Bioquímica Vegetal y Fotosíntesis, Centro de Investigaciones Científicas Isla de la Cartuja, Universidad de Sevilla-CSIC, c/Americo Vespucio No. 49, 41092-Sevilla, Spain
2Department of Biochemistry, Michigan State University, East Lansing, MI 48824, USA

*Corresponding author: E-mail, jmromero{at}ibvf.csic.es; Fax, + 34-954460065.


  Abstract

In higher plants, ADP-glucose pyrophosphorylase (ADPGlc-PPase) is a heterotetrameric enzyme comprised of two small and two large subunits. Potato–Arabidopsis hybrid ADPGlc-PPases were generated and their regulatory properties analyzed. We show that ADPGlc-PPase subunits from two different species can interact, producing active enzymes with new regulatory properties. Depending on the subunit combinations, hybrid heterotetramers showed responses to allosteric effectors [3-phosphoglycerate (3-PGA) and Pi] in the micromolar or millimolar range. While hybrid potato small subunit (PSS) and the Arabidopsis large subunit APL1 showed an extremely sensitive response to 3-PGA and Pi, hybrid PSS/Arabidopsis APL2 was very insensitive to them. Intermediate responses were determined for other subunit combinations.

Keywords: ADP-glucose pyrophosphorylase - Allosteric regulation - Arabidopsis thaliana - Heterotetrameric enzyme - Hybrid enzyme - Potato

Abbreviations: ADPGlc, ADP-glucose; ADPGlc-PPase, ADP-glucose pyrophosphorylase; APL, Arabidopsis ADPGlc-PPase large subunit; APS, Arabidopsis ADPGlc-PPase small subunit; BSA, bovine serum albumin; Glc-1-P, glucose-1-phosphate; L subunit, ADPGlc-PPase large subunit; 3-PGA, 3-phosphoglycerate; PLS, potato tuber ADPGlc-PPase large subunit; PSS, potato tuber ADPGlc-PPase small subunit; S subunit, ADPGlc-PPase small subunit.

3Present address: Department of Chemistry, Loyola University Chicago. 405 Flanner Hall, 1068 W. Sheridan Road, Chicago, IL 60626, USA.

4Present address: Department of Cell and Development Biology, John Innes Centre, Norwich Research Park, Norwich NR4 7UH, UK.

(Received March 21, 2007; Accepted April 16, 2007)
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T. Ventriglia, M. L. Kuhn, M. T. Ruiz, M. Ribeiro-Pedro, F. Valverde, M. A. Ballicora, J. Preiss, and J. M. Romero
Two Arabidopsis ADP-Glucose Pyrophosphorylase Large Subunits (APL1 and APL2) Are Catalytic
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