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Plant and Cell Physiology Advance Access originally published online on February 27, 2007
Plant and Cell Physiology 2007 48(4):648-654; doi:10.1093/pcp/pcm029
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© The Author 2007. Published by Oxford University Press on behalf of Japanese Society of Plant Physiologists. All rights reserved. For permissions, please email: journals.permissions@oxfordjournals.org

Short Communication

Two Alternatively Spliced Transcripts Generated from OsMUS81, a Rice Homolog of Yeast MUS81, Are Up-Regulated by DNA-Damaging Treatments

Naozumi Mimida1,3, Hiroko Kitamoto1,4, Keishi Osakabe1, Marina Nakashima1, Yuji Ito1,5, Wolf-Dietrich Heyer2, Seiichi Toki1 and Hiroaki Ichikawa1,*

1National Institute of Agrobiological Sciences, 2-1-2 Kannondai, Tsukuba, 305-8602 Japan
2Section of Microbiology and Section of Molecular and Cellular Biology, Center for Genetics and Development, University of California, Davis, CA 95616-8665, USA

*Corresponding author: E-mail, hichkw{at}affrc.go.jp; Fax, +81-29-838-7073.


  Abstract

OsMUS81, a rice homolog of the yeast MUS81 endonuclease gene, produced two alternative transcripts, OsMUS81{alpha} and OsMUS81ß. OsMus81{alpha} contained a Helix–hairpin–Helix (HhH) motif at the N- and C-termini, and a conserved XPF-like motif in the center, while the OsMus81ß isoform lacked the second HhH motif by alternative splicing of a cryptic intron generating a truncated protein. The two transcripts were induced after DNA-damaging treatments such as high intensity light, UV-C and {gamma}-radiation. The yeast two-hybrid assay detected a strong interaction between OsMus81 and OsRad54 recombinational repair proteins. These findings suggest that OsMus81 functions in maintaining genome integrity through homologous recombination.

Keywords: Alternative splicing - DNA repair - Homologous recombination - MUS81 - Rice - XPF endonuclease superfamily

Abbreviations: HhH, Helix–hairpin–Helix; HJ, Holliday junction; HR, homologous recombination; MMR, mismatch repair; MMS, methylmethane sulfonate; NER, nucleotide excision repair; ORF, open reading frame; RF, replication fork; RT–PCR, reverse transcription–PCR

3 Present address: Department of Apple Research, National Institute of Fruit Tree Science, 92 Nabe-yashiki, Shimo-kuriyagawa, Morioka, 020-0123 Japan.

4 Present address: Environmental Biofunction Division, National Institute for Agro-Environmental Sciences, 3-1-3 Kannondai, Tsukuba, Ibaraki, 305-8604 Japan.

5 Present address: National Agricultural Research Center for Hokkaido Region, 1 Hitsujigaoka, Toyohira-ku, Sapporo, Hokkaido, 062-8555 Japan.

(Received September 29, 2006; Accepted February 20, 2007)
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