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Plant and Cell Physiology Advance Access originally published online on February 6, 2007
Plant and Cell Physiology 2007 48(3):451-458; doi:10.1093/pcp/pcm015
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© The Author 2007. Published by Oxford University Press on behalf of Japanese Society of Plant Physiologists. All rights reserved. For permissions, please email: journals.permissions@oxfordjournals.org

Large-Scale Analysis of Chlorophyll Fluorescence Kinetics in Synechocystis sp. PCC 6803: Identification of the Factors Involved in the Modulation of Photosystem Stoichiometry

Hiroshi Ozaki1, Masahiko Ikeuchi2, Teruo Ogawa3, Hideya Fukuzawa4 and Kintake Sonoike1,*

1Department of Integrated Biosciences, Graduate School of Frontier Sciences, The University of Tokyo, 5-1-5 Kashiwanoha, Kashiwa-shi, Chiba, 277-8562 Japan
2Department of Life Sciences, The University of Tokyo, Komaba 3-8-1, Meguro, Tokyo, 153-8902 Japan
3Bioscience Center, Nagoya University, Chikusa, Nagoya, 464-8601 Japan
4Division of Integrated Life Science, Graduate School of Biostudies, Kyoto University, Kitashirakawa Oiwake-cho, Kyoto, 606-8502 Japan

*Corresponding author: E-mail, sonoike{at}k.u-tokyo.ac.jp; Fax, +81-4-7136-3651.


  Abstract

Since chlorophyll fluorescence reflects the redox state of photosynthetic electron transport chain, monitoring of chlorophyll fluorescence has been successfully applied for the screening of photosynthesis-related genes. Here we report that the mutants having a defect in the regulation of photosystem stoichiometry could be identified through the simple comparison of the induction kinetics of chlorophyll fluorescence. We made a library containing 500 mutants in the cyanobacterium Synechocystis sp. PCC 6803 with transposon-mediated gene disruption, and the mutants were used for the measurement of chlorophyll fluorescence kinetics for 45 s. We picked up two genes, pmgA and sll1961, which are involved in the modulation of photosystem stoichiometry. The disruptants of the two genes share common characteristics in their fluorescence kinetics, and we searched for mutants that showed such characteristics. Out of six mutants identified so far, five showed a different photosystem stoichiometry under high-light conditions. Thus, categorization based on the similarity of fluorescence kinetics is an excellent way to identify the function of genes.

Keywords: Chlorophyll fluorescence - Cyanobacteria - Gene function - Mutant library - Photosystem stoichiometry

Abbreviations: ORF, open reading frame

(Received December 8, 2006; Accepted January 25, 2007)
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