Skip Navigation


Plant and Cell Physiology Advance Access originally published online on January 4, 2007
Plant and Cell Physiology 2007 48(2):345-361; doi:10.1093/pcp/pcm001
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Supplementary data
Right arrow All Versions of this Article:
48/2/345    most recent
pcm001v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (17)
Right arrowRequest Permissions
Google Scholar
Right arrow Articles by Romagnoli, S.
Right arrow Articles by Cresti, M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Romagnoli, S.
Right arrow Articles by Cresti, M.
Agricola
Right arrow Articles by Romagnoli, S.
Right arrow Articles by Cresti, M.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

© The Author 2007. Published by Oxford University Press on behalf of Japanese Society of Plant Physiologists. All rights reserved. For permissions, please email: journals.permissions@oxfordjournals.org

Microtubule- and Actin Filament-Dependent Motors are Distributed on Pollen Tube Mitochondria and Contribute Differently to Their Movement

Silvia Romagnoli1, Giampiero Cai1, Claudia Faleri1, Etsuo Yokota2, Teruo Shimmen2 and Mauro Cresti1,*

1Dipartimento Scienze Ambientali ‘G. Sarfatti’, Università di Siena, via Mattioli 4, I-53100 Siena, Italy
2Department of Life Science, Faculty of Science, University of Hyogo, Harima Science Park City, Hyogo, 678-12 Japan

*Corresponding author: E-mail, cresti{at}unisi.it; Fax, +39-0577-232860.


  Abstract

The pollen tube exhibits cytoplasmic streaming of organelles, which is dependent on the actin–myosin system. Although microtubule-based motors have also been identified in the pollen tube, many uncertainties exist regarding their role in organelle transport. As part of our attempt to understand the role of microtubule-based movement in the pollen tube of tobacco, we investigated the cooperation between microtubules and actin filaments in the transport of mitochondria and Golgi vesicles, which are distributed differently in the growing pollen tube. The analysis was performed using in vitro motility assays in which organelles move along both microtubules and actin filaments. The results indicated that the movement of mitochondria and Golgi vesicles is slow and continuous along microtubules but fast and irregular along actin filaments. In addition, the presence of microtubules in the motility assays forces organelles to use lower velocities. Actin- and tubulin-binding tests, immunoblotting and immunogold labeling indicated that different organelles bind to identical myosins but associate with specific kinesins. We found that a 90 kDa kinesin (previously known as 90 kDa ATP-MAP) is associated with mitochondria but not with Golgi vesicles, whereas a 170 kDa myosin is distributed on mitochondria and other organelle classes. In vitro and in vivo motility assays indicate that microtubules and kinesins decrease the speed of mitochondria, thus contributing to their positioning in the pollen tube.

Keywords: Kinesin - Microtubule - Mitochondria - Myosin - Organelle movement - Pollen tube

Abbreviations: DIC, differential interference contrast; DTT, dithiothreitol; PMSF, phenylmethylsulfonyl fluoride; PNS, post-nuclear supernatant

(Received November 24, 2006; Accepted December 30, 2006)
Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Plant Cell PhysiolHome page
Md. S. Islam, Y. Niwa, and S. Takagi
Light-Dependent Intracellular Positioning of Mitochondria in Arabidopsis thaliana Mesophyll Cells
Plant Cell Physiol., June 1, 2009; 50(6): 1032 - 1040.
[Abstract] [Full Text] [PDF]

Home page
 Plant Cell PhysiolHome page
T. Hamada, H. Igarashi, R. Taguchi, M. Fujiwara, Y. Fukao, T. Shimmen, E. Yokota, and S. Sonobe
The Putative RNA-Processing Protein, THO2, is a Microtubule-Associated Protein in Tobacco
Plant Cell Physiol., April 1, 2009; 50(4): 801 - 811.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
G. Cai and M. Cresti
Organelle motility in the pollen tube: a tale of 20 years
J. Exp. Bot., February 1, 2009; 60(2): 495 - 508.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
E. Yokota, S. Ueda, K. Tamura, H. Orii, S. Uchi, S. Sonobe, I. Hara-Nishimura, and T. Shimmen
An isoform of myosin XI is responsible for the translocation of endoplasmic reticulum in tobacco cultured BY-2 cells
J. Exp. Bot., January 1, 2009; 60(1): 197 - 212.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
K. Hashimoto, H. Igarashi, S. Mano, C. Takenaka, T. Shiina, M. Yamaguchi, T. Demura, M. Nishimura, T. Shimmen, and E. Yokota
An isoform of Arabidopsis myosin XI interacts with small GTPases in its C-terminal tail region
J. Exp. Bot., October 1, 2008; 59(13): 3523 - 3531.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
J. Bove, B. Vaillancourt, J. Kroeger, P. K. Hepler, P. W. Wiseman, and A. Geitmann
Magnitude and Direction of Vesicle Dynamics in Growing Pollen Tubes Using Spatiotemporal Image Correlation Spectroscopy and Fluorescence Recovery after Photobleaching
Plant Physiology, August 1, 2008; 147(4): 1646 - 1658.
[Abstract] [Full Text] [PDF]

Home page
 Mol PlantHome page
A. Y. Cheung, Q.-h. Duan, S. S. Costa, B. H.J. de Graaf, V. S. Di Stilio, J. Feijo, and H.-M. Wu
The Dynamic Pollen Tube Cytoskeleton: Live Cell Studies Using Actin-Binding and Microtubule-Binding Reporter Proteins
Mol Plant, July 1, 2008; 1(4): 686 - 702.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
I. A. Sparkes, N. A. Teanby, and C. Hawes
Truncated myosin XI tail fusions inhibit peroxisome, Golgi, and mitochondrial movement in tobacco leaf epidermal cells: a genetic tool for the next generation
J. Exp. Bot., June 1, 2008; 59(9): 2499 - 2512.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
J.-F. Li and A. Nebenfuhr
Organelle Targeting of Myosin XI Is Mediated by Two Globular Tail Subdomains with Separate Cargo Binding Sites
J. Biol. Chem., July 13, 2007; 282(28): 20593 - 20602.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.