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Plant and Cell Physiology Advance Access originally published online on January 6, 2007
Plant and Cell Physiology 2007 48(2):310-321; doi:10.1093/pcp/pcm004
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© The Author 2007. Published by Oxford University Press on behalf of Japanese Society of Plant Physiologists. All rights reserved. For permissions, please email: journals.permissions@oxfordjournals.org

The Expression Patterns of Three Class B Genes in Two Distinctive Whorls of Petaloid Tepals in Alstroemeria ligtu

Masayo Hirai, Taichi Kamimura and Akira Kanno*

Graduate School of Life Sciences, Tohoku University, Katahira 2-1-1, Aoba-ku, Sendai, Miyagi, 980-8577 Japan

*Corresponding author: E-mail, kanno{at}ige.tohoku.ac.jp; Fax, +81-22-217-5725.


  Abstract

Alstroemeria (Liliales) has two layers of petaloid tepals, in which the often spotted narrow inner tepals can be distinguished easily from the wider outer tepals. In order to explore this floral morphology in Alstroemeria, we investigated the tepal morphology and the expression patterns of three class B genes, whose homologs in eudicots have been shown previously to be involved in petal and stamen development. The two DEF-like genes (AlsDEFa and AlsDEFb) and the one GLO-like gene (AlsGLO) of Alstroemeria ligtu were isolated by rapid amplification of cDNA ends (RACE). Northern hybridization, reverse transcription–PCR (RT–PCR) and in situ hybridization analyses indicated that AlsDEFb and AlsGLO were expressed in whorls 1, 2 and 3 (outer tepals, inner tepals and stamens, respectively), whereas AlsDEFa expression was detected only in whorls 2 and 3. These results suggest that in A. ligtu, AlsDEFb and AlsGLO would participate in determining the organ identity of the two-layered petaloid tepals and stamens, which is in support of the modified ABC model. Additionally, the distinctive expression patterns of AlsDEFa and AlsDEFb might be related to morphological differences between the two-layered tepals.

Keywords: ABC model - Alstroemeria - Class B gene - Floral development - MADS-box gene

Abbreviations: DIG, digoxigenin; RACE, rapid amplification of cDNA ends; RT–PCR, reverse transcription–PCR; SEM, scanning electron microscope

(Received November 17, 2006; Accepted December 26, 2006)
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Compiled by, F. Tooke, T. Chiurugwi, and N. Battey
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