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Plant and Cell Physiology Advance Access originally published online on August 30, 2007
Plant and Cell Physiology 2007 48(10):1404-1413; doi:10.1093/pcp/pcm115
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© The Author 2007. Published by Oxford University Press on behalf of Japanese Society of Plant Physiologists. All rights reserved. For permissions, please email: journals.permissions@oxfordjournals.org

Comprehensive Analysis of Glucan Elicitor-Regulated Gene Expression in Tobacco BY-2 Cells Reveals a Novel MYB Transcription Factor Involved in the Regulation of Phenylpropanoid Metabolism

Tomonori Shinya1,2,4,*, Ivan Gális2,5, Tomoko Narisawa2, Mami Sasaki2, Hiroo Fukuda2, Hideaki Matsuoka1, Mikako Saito1 and Ken Matsuoka2,3

1Department of Biotechnology and Life Science, Tokyo University of Agriculture and Technology, 2-24-16, Naka-cho, Koganei, Tokyo, 184-8588 Japan
2RIKEN Plant Science Center, 1-7-22, Suehiro-cho, Tsurumi-ku, Yokohama, 230-0045 Japan and
3Laboratory of Plant Nutrition, Faculty of Agriculture, Kyushu University, 6-10-1, Hakozaki, Higashi-ku, Fukuoka, 812-8581 Japan

*Corresponding author: E-mail, shinya{at}isc.meiji.ac.jp; Fax, +81-44-934-7039.


  Abstract

We previously demonstrated that a ß-1,3-, 1,6-oligoglucan (AaGlucan) from the fungus Alternaria alternata 102 shows strong elicitor activity in tobacco BY-2 cells. We have used cDNA microarray analysis to monitor global changes in gene expression in tobacco cells treated with this A. alternata fraction or with laminarin. In total, we identified 265 genes that were induced 1 h after treatment with an AaGlucan-enriched fraction or laminarin. Among them, we characterized in detail a novel tobacco R2R3 MYB-type transcription factor homolog (NtMYBGR1) and two DC1 domain-containing genes (NtDC1A and NtDC1B). Microarray data, together with overexpression and metabolic analyses, indicated that NtMYBGR1, but not the NtDC1 proteins, primarily targets the phenylpropanoid synthesis-related genes PAL and 4CL. These results suggest that NtMYBGR1 specifically regulates defense responses in BY-2 cells by enhancing phenylpropanoid metabolism in response to AaGlucan and laminarin elicitors.

Keywords: BY-2 cells - Gene expression - Glucan elicitor - Microarray - MYB - Phenylpropanoid pathway

Abbreviations: CaMV, cauliflower mosaic virus; CaPU, caffeoylputrescine; CCoAOMT, caffeoyl-CoA O-methyltransferase; 4CL, 4-coumarate:CoA ligase; C4H, cinnamate 4-hydroxylase; EST, expressed sequence tag; FePU, feruloylputrescine; MeJA, methyl jasmonate; ORF, open reading frame; PAL, phenylalanine ammonia-lyase; PAMPs, pathogen-associated molecular patterns; RT–PCR, reverse transcription–PCR

4Present address: Department of Life Sciences, Faculty of Agriculture, Meiji University, Kawasaki, 214-8571 Japan.

5Present address: Department of Molecular Ecology, Max-Planck-Institute for Chemical Ecology, D-07745 Jena, Germany.

(Received May 21, 2007; Accepted August 10, 2007)
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