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Plant and Cell Physiology Advance Access originally published online on December 5, 2006
Plant and Cell Physiology 2007 48(1):186-192; doi:10.1093/pcp/pcl050
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© The Author 2006. Published by Oxford University Press on behalf of Japanese Society of Plant Physiologists. All rights reserved. For permissions, please email: journals.permissions@oxfordjournals.org

Short Communication

Two Novel Nuclear Genes, OsSIG5 and OsSIG6, Encoding Potential Plastid Sigma Factors of RNA Polymerase in Rice: Tissue-Specific and Light-Responsive Gene Expression

Yoshiki Kubota1, Akio Miyao2, Hirohiko Hirochika2, Yuzuru Tozawa3, Hiroyuki Yasuda4, Yuichi Tsunoyama4, Yasuo Niwa5, Sousuke Imamura1, Makoto Shirai1 and Munehiko Asayama1,*

1Laboratory of Molecular Genetics, Collage of Agriculture, Ibaraki University, Ami, Inashiki, Ibaraki, 300-0393 Japan
2Department of Molecular Genetics, National Institute of Agrobiological Resources, Tsukuba, Ibaraki 305-602, Japan
3Division of Biomolecular Engineering, Cell-Free Science and Technology Research Center, Ehime University, Bunkyo-cho 3, Matsuyama, Ehime, 790-8577 Japan
4Radioisotope Research Center, Kyoto University, Kitashirakawa-oiwake-cho, Sakyo-ku, Kyoto, 606-8502 Japan
5Laboratory of Plant Cell Technology, Graduate School of Nutritional and Environment Science, University of Shizuoka, 52-1 Yada, Shizuoka, 422 Japan

*Corresponding author: E-mail, asam{at}mx.ibaraki.ac.jp; Fax, +81-29-888-8651.


  Abstract

Two novel nuclear genes, OsSIG5 and OsSIG6, encoding potential plastid sigma factors of RNA polymerase (RNAP) were identified in Oryza sativa. The deduced amino acid sequences contain conserved regions, regions 1.2–4.2, and a novel region A/B at the N-terminus. Tissue-specific and light-responsive transcripts of OsSIG5 and OsSIG6 were observed. The N-terminal region of OsSig5 conferred import of green fluorescent protein into the chloroplast. Specific transcripts of rice psbA were synthesized in vitro by reconstituted OsSig5–RNAP holoenzymes. These results indicated that OsSig5 is a plastid sigma factor. This is the first report of the Sig5-type sigma factor in crops.

Keywords: Blue/red-light responsive expression - Chloroplast RNA polymerase - Oryza sativa - Promoter - Sigma factor - Transcription

Abbreviations: GFP, green fluorescence protein; psbA, the gene encoding protein D1 of photosystem II; psbD, the gene encoding protein D2 of photosystem II; RNAP, RNA polymerase; RT–PCR, reverse transcription–PCR; TP, transit peptide

The nucleotide sequences reported in this paper have been submitted to DDBJ under accession numbers AB096011/AB096071 (OsSIG5) and AB096012 (OsSIG6).

(Received October 9, 2006; Accepted November 27, 2006)
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