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Plant and Cell Physiology Advance Access originally published online on December 12, 2006
Plant and Cell Physiology 2007 48(1):169-178; doi:10.1093/pcp/pcl052
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© The Author 2006. Published by Oxford University Press on behalf of Japanese Society of Plant Physiologists. All rights reserved. For permissions, please email: journals.permissions@oxfordjournals.org

Isolation of 151 Mutants that Have Developmental Defects from T-DNA Tagging

Ji Hoon Ahn1,3,*, Joonki Kim1,3, Seong Jeon Yoo1, So Yeon Yoo1, Hyungmin Roh2, Jun-Hyuk Choi2,4, Mi Suk Choi1,4, Kyung Sook Chung1,4, Eun Ju Han2,4, Sung Myun Hong1,4, Sung Hye Jung1,4, Hyo Jin Kang2,4, Bo Kyung Kim2,4, Mi Duk Kim2,4, Youn Kyung Kim2,4, Yun Hee Kim2,4, Hanna Lee1,4, Soo Hyun Park1,4, Jee Hoon Yang1,4, Jung Won Yang2,4, Dong-Hun Yoo2,4, Seung Kwan Yoo1,4 and Jong Seob Lee2,*

1Plant Signaling Network Research Center, School of Life Sciences and Biotechnology, Korea University, Seoul 136-701, Korea
2School of Biological Sciences, Seoul National University, Seoul 152-742, Korea

*Corresponding authors: Jong Seob Lee, E-mail, jongslee{at}plaza.snu.ac.kr; Fax, +82-2-872-1993; Ji Hoon Ahn, E-mail, jahn{at}korea.ac.kr; Fax, +82-2-927-9028.


  Abstract

In order to understand the mechanisms underlying plant development, a necessary first step involves the elucidation of the functions of the genes, via the analysis of mutants that exhibit developmental defects. In this study, an activation tagging mutant library harboring 80,650 independent Arabidopsis transformants was generated in order to screen for developmental mutants. A total of 129 mutants manifesting dominant developmental abnormalities were isolated, and their T-DNA insertion loci were mapped. The activation of one or more genes adjacent to a T-DNA insertion locus was confirmed in eight dominant mutants. A gene adjacent to the right border was usually activated by the 35S enhancers. Interestingly, the transcriptional activation of multiple genes within a broad range was observed in one of the mutants, which raises the possibility that activation by the 35S enhancers was not limited strictly to a single gene. In order to gain a better understanding of sexual reproduction in higher plants, we isolated 22 mutants exhibiting defects in female gametophyte development, and determined their T-DNA insertion loci. We propose that this mutant population may prove useful in the further determination of the functions of genes that play important roles in plant development.

Keywords: Activation tagging - Arabidopsis - Developmental mutants - Female gametophytic mutant - Plant development

Abbreviations: BAC, bacterial artificial chromosome; LB, left border; RB, right border; RT–PCR, reverse transcription–PCR; TAIL-PCR, thermal asymmetric interlaced-PCR.

3 These authors contributed equally to this work.

4 These authors contributed equally to this work and are listed in alphabetical order.

(Received August 14, 2006; Accepted November 29, 2006)
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