Skip Navigation


Plant and Cell Physiology Advance Access originally published online on December 13, 2006
Plant and Cell Physiology 2007 48(1):147-158; doi:10.1093/pcp/pcl046
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
48/1/147    most recent
pcl046v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (5)
Right arrowRequest Permissions
Google Scholar
Right arrow Articles by Talbot, M. J.
Right arrow Articles by McCurdy, D. W.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Talbot, M. J.
Right arrow Articles by McCurdy, D. W.
Agricola
Right arrow Articles by Talbot, M. J.
Right arrow Articles by McCurdy, D. W.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

© The Author 2006. Published by Oxford University Press on behalf of Japanese Society of Plant Physiologists. All rights reserved. For permissions, please email: journals.permissions@oxfordjournals.org

Cellulose Synthesis is Required for Deposition of Reticulate Wall Ingrowths in Transfer Cells

Mark J. Talbot1,3, Geoffrey O. Wasteneys2,4, Christina E. Offler1 and David W. McCurdy1,*

1School of Environmental and Life Sciences, The University of Newcastle, Newcastle, NSW 2308, Australia
2Plant Cell Biology Group, Research School of Biological Sciences, The Australian National University, Canberra ACT 2601, Australia

*Corresponding author: E-mail, David.McCurdy{at}newcastle.edu.au; Fax, +61-2-49-21-6923.


  Abstract

Despite the recognized physiological importance of transfer cells, little is known about how these specialized cells achieve localized deposition of cell wall material, leading to amplification of plasma membrane surface area and enhanced membrane transport capacity. This study establishes that cellulose synthesis is a key early factor in the construction of ‘reticulate’ wall ingrowths, an elaborate but common form of localized wall deposition characteristic of most transfer cells. Using field emission scanning electron microscopy, wall ingrowths were first visible in epidermal transfer cells of Faba bean cotyledons as raised ‘patches’ of disorganized and tangled cellulosic material, and, from these structures, ingrowths emerged via further deposition of wall material. The cellulose biosynthesis inhibitors 2,6-dichlorobenzonitrile and isoxaben both caused dramatic reductions in the number of cells depositing wall ingrowths, altered wall ingrowth morphology and visibly disrupted microfibril structure. The restriction of cellulose deposition to discrete patches suggests a novel mechanism for cellulose synthesis in this circumstance. Overall, these results implicate a central role for cellulose synthesis in reticulate wall ingrowth morphology, especially at the initial stage of ingrowth formation, possibly by providing a template for the self-assembly of wall polymers.

Keywords: Cellulose microfibril - Field emission scanning electron microscopy - Transfer cell - Vicia faba - Wall ingrowth

Abbreviations: DCB, 2,6-dichlorobenzonitrile; DMSO, dimethylsulfoxide; FESEM, field emission scanning electron microscopy; SEM, scanning electron microscopy; TEM, transmission electron microscopy.

3 Present address: Microscopy Unit, CSIRO Plant Industry, Canberra, ACT, 2601, Australia

4 Present address: Department of Botany, University of British Columbia, 3529–6270 University Boulevard, Vancouver, British Columbia, Canada V6T 1Z4

(Received September 13, 2006; Accepted November 25, 2006)
Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Plant CellHome page
H. Maeda, T. L. Sage, G. Isaac, R. Welti, and D. DellaPenna
Tocopherols Modulate Extraplastidic Polyunsaturated Fatty Acid Metabolism in Arabidopsis at Low Temperature
PLANT CELL, February 1, 2008; 20(2): 452 - 470.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.