Skip Navigation


Plant and Cell Physiology Advance Access originally published online on December 13, 2006
Plant and Cell Physiology 2007 48(1):122-133; doi:10.1093/pcp/pcl053
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
48/1/122    most recent
pcl053v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrowRequest Permissions
Google Scholar
Right arrow Articles by Dunn, E. K.
Right arrow Articles by Mandoli, D. F.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Dunn, E. K.
Right arrow Articles by Mandoli, D. F.
Agricola
Right arrow Articles by Dunn, E. K.
Right arrow Articles by Mandoli, D. F.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

© The Author 2006. Published by Oxford University Press on behalf of Japanese Society of Plant Physiologists. All rights reserved. For permissions, please email: journals.permissions@oxfordjournals.org

Spectroscopic and Biochemical Analysis of Regions of the Cell Wall of the Unicellular ‘Mannan Weed’, Acetabularia acetabulum

Erin K. Dunn1,5, Douglas A. Shoue2,5, Xuemei Huang3, Raymond E. Kline4, Alex L. MacKay3, Nicholas C. Carpita2, Iain E.P. Taylor4 and Dina F. Mandoli1,*

1Department of Biology, Center for Developmental Biology & Institute for Stem Cell and Regenerative Medicine, Box 35325, University of Washington, Seattle, WA 98195-5325, USA
2Department of Botany and Plant Pathology, Purdue University, W. Lafayette, Indiana 47907-2054, USA
3Department of Physics and Astronomy, University of British Columbia, Vancouver, BC, Canada V6T 1Z1
4Department of Botany, University of British Columbia, Vancouver, BC, Canada V6T 1Z4

*Corresponding author: E-mail, mandoli{at}u.washington.edu; Fax, +1–206–685–1728.


  Abstract

Although the Dasycladalean alga Acetabularia acetabulum has long been known to contain mannan-rich walls, it is not known to what extent wall composition varies as a function of the elaborate cellular differentiation of this cell, nor has it been determined what other polysaccharides accompany the mannans. Cell walls were prepared from rhizoids, stalks, hairs, hair scars, apical septa, gametophores and gametangia, subjected to nuclear magnetic resonance and Fourier transform infrared spectroscopy, and analyzed for monosaccharide composition and linkage, although material limitations prevented some cell regions from being analyzed by some of the methods. In diplophase, walls contain a para-crystalline mannan, with other polysaccharides accounting for 10–20% of the wall mass; in haplophase, gametangia have a cellulosic wall, with mannans and other polymers representing about a quarter of the mass. In the walls of the diplophase, the mannan appears less crystalline than typical of cellulose. The walls of both diploid and haploid phases contain little if any xyloglucan or pectic polysaccharides, but appear to contain small amounts of a homorhamnan, galactomannans and glucogalactomannans, and branched xylans. These ancillary polysaccharides are approximately as abundant in the cellulose-rich gametangia as in the mannan-rich diplophase. In the diplophase, different regions of the cell differ modestly but reproducibly in the composition of the cell wall. These results suggest unique cell wall architecture for the mannan-rich cell walls of the Dasycladales.

Keywords: Acetabularia acetabulum - Cellulose - Dasycladales - FTIR - Linkage analysis - Mannan weed

Abbreviations: FTIR, Fourier transform infrared spectroscopy; GC–MS, gas chromatography–mass spectrometry; GalA, galacturonic acid; GlcA, glucuronic acid; ManA, mannuronic acid; NMR, nuclear magnetic resonance; RG I, rhamnogalacturonan I; TFA, trifluoracetic acid.

5 Present address: c/o University of California San Diego, Biology Graduate Student Affairs, 0348, 9500 Gilman Drive, La Jolla, CA 92093-0348, USA.

(Received September 5, 2006; Accepted November 21, 2006)
Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?




Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.