Skip Navigation


Plant and Cell Physiology Advance Access originally published online on March 23, 2006
Plant and Cell Physiology 2006 47(6):689-697; doi:10.1093/pcp/pcj038
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
47/6/689    most recent
pcj038v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (21)
Right arrowRequest Permissions
Google Scholar
Right arrow Articles by Saito, S.
Right arrow Articles by Kawakita, K.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Saito, S.
Right arrow Articles by Kawakita, K.
Agricola
Right arrow Articles by Saito, S.
Right arrow Articles by Kawakita, K.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?


Peroxynitrite Generation and Tyrosine Nitration in Defense Responses in Tobacco BY-2 Cells

Syuhei Saito1, Ayako Yamamoto-Katou1,2, Hirofumi Yoshioka1, Noriyuki Doke1 and Kazuhito Kawakita1,*

1 Plant Pathology Laboratory, Graduate School of Bioagricultural Sciences, Nagoya University, Chikusa, Nagoya, 464-8601 Japan
2 Department of Molecular Genetics, National Institute of Agrobiological Resources, Tsukuba, Ibaraki, 305-8602 Japan

* Corresponding author: E-mail, kkawakit{at}agr.nagoya-u.ac.jp; Fax, +81-52-789-5525.

Peroxynitrite (ONOO–) is a compound formed by reaction of superoxide (O2 –) with nitric oxide (NO) and is expected to possess characteristics of both O2 – reactivity and NO mobility in order to function as a signal molecule. Although there are several reports that describe the role of ONOO– in defense responses in plants, it has been very difficult to detect ONOO– in bioimaging due to its short half-life or paucity of methods for ONOO–-specific detection among reactive oxygen species or free radicals. Aminophenyl fluorescein (APF), a recently developed novel fluorophore for direct detection of ONOO– in bioimaging, was used for intracellular ONOO– detection. ONOO– generation in tobacco BY-2 cells treated with INF1, the major elicitin secreted by the late blight pathogen Phytophthora infestans, occurred within 1 h and reached a maximum level at 6–12 h after INF1 treatment. Urate, a ONOO– scavenger, abolished INF1-induced ONOO– generation. It is well known that ONOO– reacts with tyrosine residues in proteins to form nitrotyrosine in a nitration reaction as an ONOO–-specific reaction. Western blot analysis using anti-nitrotyrosine antibodies recognized nitrotyrosine-containing proteins in 20 and 50 kDa bands in BY-2 protein extract containing SIN-1 [3-(4-morpholinyl) sydnonimine hydrochloride; an ONOO– donor]. These bands were also recognized in INF1-treated BY-2 cells and were found to be slightly suppressed by urate. Our study is the first to report ONOO– detection and tyrosine nitration in defense responses in plants.

(Received December 12, 2005; Accepted March 16, 2006)
Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 J Exp BotHome page
M. Chaki, R. Valderrama, A. M. Fernandez-Ocana, A. Carreras, J. Lopez-Jaramillo, F. Luque, J. M. Palma, J. R. Pedrajas, J. C. Begara-Morales, B. Sanchez-Calvo, et al.
Protein targets of tyrosine nitration in sunflower (Helianthus annuus L.) hypocotyls
J. Exp. Bot., November 1, 2009; 60(15): 4221 - 4234.
[Abstract] [Full Text] [PDF]

Home page
 Plant Cell PhysiolHome page
M. Chaki, A. M. Fernandez-Ocana, R. Valderrama, A. Carreras, F. J. Esteban, F. Luque, M. V. Gomez-Rodriguez, J. C. Begara-Morales, F. J. Corpas, and J. B. Barroso
Involvement of Reactive Nitrogen and Oxygen Species (RNS and ROS) in Sunflower-Mildew Interaction
Plant Cell Physiol., March 1, 2009; 50(3): 665 - 679.
[Abstract] [Full Text] [PDF]

Home page
 Plant Cell PhysiolHome page
M. Chaki, A. M. Fernandez-Ocana, R. Valderrama, A. Carreras, F. J. Esteban, F. Luque, M. V. Gomez-Rodriguez, J. C. Begara-Morales, F. J. Corpas, and J. B. Barroso
Involvement of Reactive Nitrogen and Oxygen Species (RNS and ROS) in Sunflower-Mildew Interaction
Plant Cell Physiol., February 1, 2009; 50(2): 265 - 279.
[Abstract] [Full Text] [PDF]

Home page
 Plant Cell PhysiolHome page
F. J. Corpas, M. Chaki, A. Fernandez-Ocana, R. Valderrama, J. M. Palma, A. Carreras, J. C. Begara-Morales, M. Airaki, L. A del Rio, and J. B. Barroso
Metabolism of Reactive Nitrogen Species in Pea Plants Under Abiotic Stress Conditions
Plant Cell Physiol., November 1, 2008; 49(11): 1711 - 1722.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
S. Asai, K. Ohta, and H. Yoshioka
MAPK Signaling Regulates Nitric Oxide and NADPH Oxidase-Dependent Oxidative Bursts in Nicotiana benthamiana
PLANT CELL, May 1, 2008; 20(5): 1390 - 1406.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
S. Neill, R. Barros, J. Bright, R. Desikan, J. Hancock, J. Harrison, P. Morris, D. Ribeiro, and I. Wilson
Nitric oxide, stomatal closure, and abiotic stress
J. Exp. Bot., February 1, 2008; 59(2): 165 - 176.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
M. C. Romero-Puertas, M. Laxa, A. Matte, F. Zaninotto, I. Finkemeier, A. M.E. Jones, M. Perazzolli, E. Vandelle, K.-J. Dietz, and M. Delledonne
S-Nitrosylation of Peroxiredoxin II E Promotes Peroxynitrite-Mediated Tyrosine Nitration
PLANT CELL, December 1, 2007; 19(12): 4120 - 4130.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.