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Plant and Cell Physiology Advance Access originally published online on March 10, 2006
Plant and Cell Physiology 2006 47(5):673-677; doi:10.1093/pcp/pcj032
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Short Communication

Plant Lanosterol Synthase: Divergence of the Sterol and Triterpene Biosynthetic Pathways in Eukaryotes

Satoru Sawai1, Tomoyoshi Akashi1, Nozomu Sakurai2, Hideyuki Suzuki2, Daisuke Shibata2, Shin-ichi Ayabe1 and Toshio Aoki1,*

1 Department of Applied Biological Sciences, Nihon University, Fujisawa, Kanagawa, 252-8510 Japan
2 Kazusa DNA Research Institute, Kisarazu, Chiba, 292-0818 Japan

* Corresponding author: E-mail: taoki{at}brs.nihon-u.ac.jp; Fax +81–466–84–3353.

Sterols, essential eukaryotic constituents, are biosynthesized through either cyclic triterpenes, lanosterol (fungi and animals) or cycloartenol (plants). The cDNA for OSC7 of Lotus japonicus was shown to encode lanosterol synthase (LAS) by the complementation of a LAS-deficient mutant yeast and structural identification of the accumulated lanosterol. A double site-directed mutant of OSC7, in which amino acid residues crucial for the reaction specificity were changed to the cycloartenol synthase (CAS) type, produced parkeol and cycloartenol. The multiple amino acid sequence alignment of a conserved region suggests that the LAS of different eukaryotic lineages emerged from the ancestral CAS by convergent evolution.

The nucleotide sequences reported in this paper have been submitted to the DDBJ/EMBL/GenBank database under accession numbers AB244670 (cOSC6) and AB244671 (cOSC7).

(Received February 21, 2006; Accepted March 7, 2006)
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