Plant and Cell Physiology Advance Access originally published online on March 9, 2006
Plant and Cell Physiology 2006 47(5):591-600; doi:10.1093/pcp/pcj026
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Overexpression of PRE1 and its Homologous Genes Activates Gibberellin-dependent Responses in Arabidopsis thaliana
1 Department of Molecular Biology, Sejong University, 98 Gunja-Dong, Gwangjin-Gu, Seoul 143-747, Republic of Korea
2 Kumho Life & Environmental Science Laboratory, 1 Oryong-Dong, Buk-Gu, Gwangju 500-712, Republic of Korea
* Corresponding author: E-mail, soh{at}sejong.ac.kr; Fax, +82-2-3408-3661.
Gibberellins control various aspects of growth and development. Here, we identified a gene, designated paclobutrazol resistance1 (PRE1), by screening Arabidopsis activation-tagged lines. PRE1 encodes a helix–loop–helix protein and belongs to a small gene family. Physiological and genetic analysis indicated that overexpression of PRE1 altered various aspects of gibberellin-dependent responses such as germination, elongation of hypocotyl/petiole, floral induction and fruit development, and suppressed gibberellin-deficient phenotypes of the ga2 mutant. Expression of some gibberellin-responsive genes was also affected by PRE1. Expression of PRE1 was shown to be early gibberellin inducible in the wild-type plants and under control of SPY and GAI, upstream negative regulators of gibberellin signaling. The shortened hypocotyl length phenotype of the gai-1 mutant was suppressed by PRE1 overexpression. Ectopic overexpression of each of the four PRE1-related genes conferred pleiotropic phenotypes similar to PRE1 overexpression, indicative of overlapping functions among the PRE gene family. Our results of gain-of-function studies suggest that PRE genes may have a regulatory role in gibberellin-dependent development in Arabidopsis thaliana.
3 Present address: Division of Applied Bioscience and Biotechnology, Chonnam National University, 300 Yongbong-dong, Buk-gu, Gwangju 500-757, South Korea
(Received January 16, 2006; Accepted February 25, 2006)
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  A. Tanaka, H. Nakagawa, C. Tomita, Z. Shimatani, M. Ohtake, T. Nomura, C.-J. Jiang, J. G. Dubouzet, S. Kikuchi, H. Sekimoto, et al. BRASSINOSTEROID UPREGULATED1, Encoding a Helix-Loop-Helix Protein, Is a Novel Gene Involved in Brassinosteroid Signaling and Controls Bending of the Lamina Joint in Rice Plant Physiology, October 1, 2009; 151(2): 669 - 680. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. J. Lee, J. W. Park, H. W. Lee, and J. Kim Genome-wide analysis of the auxin-responsive transcriptome downstream of iaa1 and its expression analysis reveal the diversity and complexity of auxin-regulated gene expression J. Exp. Bot., September 1, 2009; 60(13): 3935 - 3957. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K.-Y. Chen, B. Cong, R. Wing, J. Vrebalov, and S. D. Tanksley Changes in Regulation of a Transcription Factor Lead to Autogamy in Cultivated Tomatoes Science, October 26, 2007; 318(5850): 643 - 645. [Abstract] [Full Text] [PDF]
|
|