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Plant and Cell Physiology Advance Access originally published online on January 24, 2006
Plant and Cell Physiology 2006 47(4):549-553; doi:10.1093/pcp/pcj014
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Short Communication

An Alloplasmic Male-sterile Line of Brassica oleracea Harboring the Mitochondria from Diplotaxis muralis Expresses a Novel Chimeric Open Reading Frame, orf72

Tomotaka Shinada1, Yosuke Kikuchi1, Ryo Fujimoto and Sachie Kishitani*

Laboratory of Plant Breeding and Genetics, Graduate School of Agricultural Science, Tohoku University, 1-1 Tsutsumidori-Amamiyamachi, Aoba-ku, Sendai, 981-8555 Japan

* Corresponding author: E-mail, kishitan{at}bios.tohoku.ac.jp; Fax, +81-22-7178654.

Nuclear so-called fertility-restorer genes reverse the pollen sterility of cytoplasmic male-sterile (CMS) plants caused by disturbed mitochondrial–nuclear interactions. We identified a CMS-associated chimeric mitochondrial gene in an alloplasmic CMS line of Brassica oleracea in the ‘mur’ system. This novel chimeric gene, orf72, was found in the mitochondrial genome of donor cytoplasm. It was located downstream of normal rps7 and contained part of atp9 (atp9-b). It was expressed specifically on the nuclear background of CMS B. oleracea, partially suppressed in the fertility-restored line and entirely suppressed in the cytoplasmic donor.

The nucleotide sequences reported in this paper have been submitted to DDBJ under the following accession numbers: AB243571 [GenBank] (rps7, truncated orf188, atp9-b and orf72) and AB243572 [GenBank] (atp9-a).

1 These authors contributed equally to this work.

(Received December 16, 2005; Accepted January 19, 2006)
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