Skip Navigation


Plant and Cell Physiology Advance Access originally published online on January 13, 2006
Plant and Cell Physiology 2006 47(3):363-371; doi:10.1093/pcp/pcj002
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Supplementary Figure
Right arrow All Versions of this Article:
47/3/363    most recent
pcj002v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (17)
Right arrowRequest Permissions
Google Scholar
Right arrow Articles by Kikuchi, S.
Right arrow Articles by Nakai, M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Kikuchi, S.
Right arrow Articles by Nakai, M.
Agricola
Right arrow Articles by Kikuchi, S.
Right arrow Articles by Nakai, M.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?


Characterization of the Preprotein Translocon at the Outer Envelope Membrane of Chloroplasts by Blue Native PAGE

Shingo Kikuchi, Toshiya Hirohashi and Masato Nakai*

Institute for Protein Research, Osaka University, 3-2 Yamadaoka, Suita, Osaka, 565-0871 Japan

* Corresponding author: E-mail, nakai{at}protein.osaka-u.ac.jp; Fax, +81-6-6879-8613.

The preprotein translocon at the outer envelope membrane of chloroplasts (Toc) mediates the recognition and import of nuclear-encoded preproteins into chloroplasts. Two receptor components, Toc159 and Toc34, and the channel Toc75 form the Toc complex. In this study, we have analyzed the molecular architecture and organization of the Toc complex by blue native PAGE (BN–PAGE), which is a high-resolution method for separating membrane protein complexes under non-denaturing conditions. Pea chloroplasts isolated in the presence of a protease inhibitor cocktail were directly solubilized in detergent solution and analyzed by BN–PAGE and size exclusion chromatography. Subsequent immunoblot analyses indicated that the complex composed of Toc75, Toc159 and Toc34 has a molecular mass of 800–1,000 kDa. Limited proteolysis revealed a core of the Toc complex, which was resistant to proteases and detergent treatments. The stoichiometry of the three Toc proteins was calculated as approximately 1 : 3 : 3 between Toc159 : Toc75 : Toc34. We have also analyzed the Toc complex of etioplasts and root plastids. These plastids were found to have essentially the same sized Toc complex as that of the chloroplast.

(Received November 18, 2005; Accepted December 28, 2005)
Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Plant CellHome page
S. Kikuchi, M. Oishi, Y. Hirabayashi, D. W. Lee, I. Hwang, and M. Nakai
A 1-Megadalton Translocation Complex Containing Tic20 and Tic21 Mediates Chloroplast Protein Import at the Inner Envelope Membrane
PLANT CELL, June 1, 2009; 21(6): 1781 - 1797.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
K. Oikawa, A. Yamasato, S.-G. Kong, M. Kasahara, M. Nakai, F. Takahashi, Y. Ogura, T. Kagawa, and M. Wada
Chloroplast Outer Envelope Protein CHUP1 Is Essential for Chloroplast Anchorage to the Plasma Membrane and Chloroplast Movement
Plant Physiology, October 1, 2008; 148(2): 829 - 842.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
P. Koenig, M. Oreb, K. Rippe, C. Muhle-Goll, I. Sinning, E. Schleiff, and I. Tews
On the Significance of Toc-GTPase Homodimers
J. Biol. Chem., August 22, 2008; 283(34): 23104 - 23112.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
J. Wang, J. E. Elliott, and R. E. Williamson
Features of the primary wall CESA complex in wild type and cellulose-deficient mutants of Arabidopsis thaliana
J. Exp. Bot., July 1, 2008; 59(10): 2627 - 2637.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
M. Oreb, A. Hofle, O. Mirus, and E. Schleiff
Phosphorylation regulates the assembly of chloroplast import machinery
J. Exp. Bot., June 1, 2008; 59(9): 2309 - 2316.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
L. E. Reddick, M. D. Vaughn, S. J. Wright, I. M. Campbell, and B. D. Bruce
In Vitro Comparative Kinetic Analysis of the Chloroplast Toc GTPases
J. Biol. Chem., April 13, 2007; 282(15): 11410 - 11426.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
R. Bredemeier, T. Schlegel, F. Ertel, A. Vojta, L. Borissenko, M. T. Bohnsack, M. Groll, A. von Haeseler, and E. Schleiff
Functional and Phylogenetic Properties of the Pore-forming beta-Barrel Transporters of the Omp85 Family
J. Biol. Chem., January 19, 2007; 282(3): 1882 - 1890.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.