Plant and Cell Physiology Advance Access originally published online on December 16, 2005
Plant and Cell Physiology 2006 47(2):254-261; doi:10.1093/pcp/pci243
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Accumulation of the Two Transcripts of the N gene, Conferring Resistance to Tobacco Mosaic Virus, is Probably Important for N Gene-dependent Hypersensitive Cell Death
1 National Institute of Agrobiological Sciences (NIAS), Kannon-dai, Tsukuba, Ibaraki, 305-8602 Japan
2 National Agricultural Research Center, Tsukuba, Ibaraki, 305-8666 Japan
* Corresponding author: E-mail, yohashi{at}affrc.go.jp; Fax, +81–029–8387469.
The N gene is a Toll/interleukin-1 receptor (TIR)–nucleotide-binding site (NBS)–leucine-rich repeat (LRR)-type resistance (R) gene that generates two alternative transcripts, N S and N L. N S encodes the full-length N protein while N L is predicted to encode a truncated form of the protein lacking most of the LRR region. We found that the two transcripts were accumulated at 20°C, a permissive temperature, but not at 30°C, a non-permissive temperature for the N gene, in tobacco mosaic virus (TMV)-inoculated leaves. When N gene-dependent cell death was triggered by transient 20°C treatment for 2–6 h, considerable levels of the transcripts were accumulated just before cell death, although the levels of N S were always higher. The accumulation was induced by transient expression of the 50 kDa helicase domain (p50) of TMV replicase which is the Avr component of N, but not by transient expression of NtMEK2 DD-mediated cell death or N gene-independent hypersensitive cell death. These results suggest that the accumulation of N S and N L is associated with the function of N and, above a certain threshold, triggers N-mediated hypersensitive cell death.
(Received October 13, 2005; Accepted December 8, 2005)
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