Plant and Cell Physiology Advance Access originally published online on September 20, 2006
Plant and Cell Physiology 2006 47(11):1473-1483; doi:10.1093/pcp/pcl012
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Identification of Active Transposon dTok, a Member of the hAT Family, in Rice
1 National Research Laboratory of Plant Functional Genomics, Division of Molecular and Life Sciences, Pohang University of Science and Technology (POSTECH), Pohang 790-784, Republic of Korea
2 Laboratory of Rice Genomics and Breeding, College of Agriculture and Life Science, Seoul National University, Seoul 151-921, Republic of Korea
3 Institute of Biotechnology, Yeungnam University, Kyongsan 712-749, Republic of Korea
4 School of Biological Resources, College of Natural Resources, Yeungnam University, Kyongsan 712-749, Republic of Korea
5 Functional Genomics Center, Pohang University of Science and Technology (POSTECH), Pohang 790-784, Republic of Korea
* Corresponding author: E-mail, genean{at}postech.ac.kr; Fax, +82-54-279-0659.
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Abstract |
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Recent completion of the sequencing of the rice genome has revealed that it contains >40% repetitive sequences, most of which are related to inactive transposable elements. During the molecular analysis of the floral organ number1/multiple pistil 2 (fon1/mp2) mutant, we identified an active transposable element dTok0 that was inserted at the kinase domain of FON1, a homolog of CLAVATA1. Insertion of the element into FON1 generated an 8 bp duplication of its target sites, which is one of the major characteristics of the hAT family of transposons. The dTok0 element was actively transposed out of the FON1 gene, leaving 5–8 bp footprints. Reinsertion into a new location was observed at a low frequency. Analysis of the genome sequence showed that the rice cultivar ‘Nipponbare’ contains 25 copies of dTok elements; similar numbers were present in all the Oryza species examined. Because dTok0 does not encode a transposase, enzyme activity should be provided in trans. We identified a putative autonomous transposon, Tok1 that contains an intact open reading frame of the Ac-like transposase.
Keywords: hAT superfamily - floral organ number 1/multiple pistil 2 - Oryza - Rice - Transposon
Abbreviations: Ac, Activator; AFLP, amplified fragment length polymorphism; Ds, Dissociation; FON1, floral organ number1; MITE, miniature inverted repeat transposable element; fon1/mp2, floral organ number1/multiple pistil 2 mutant; MULE, mutator-like transposable element; ORF, open reading frame; TE, transposable element; TIR, terminal inverted repeat.
(Received June 15, 2006; Accepted September 14, 2006)
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