Skip Navigation


Plant and Cell Physiology Advance Access originally published online on August 30, 2006
Plant and Cell Physiology 2006 47(10):1412-1419; doi:10.1093/pcp/pcl003
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
47/10/1412    most recent
pcl003v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (1)
Right arrowRequest Permissions
Google Scholar
Right arrow Articles by Shih, C.-H.
Right arrow Articles by Lo, C.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Shih, C.-H.
Right arrow Articles by Lo, C.
Agricola
Right arrow Articles by Shih, C.-H.
Right arrow Articles by Lo, C.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

© The Author 2006. Published by Oxford University Press on behalf of Japanese Society of Plant Physiologists. All rights reserved. For permissions, please email: journals.permissions@oxfordjournals.org

Short Communication

Differential Expression of Two Flavonoid 3'-Hydroxylase cDNAs Involved in Biosynthesis of Anthocyanin Pigments and 3-Deoxyanthocyanidin Phytoalexins in Sorghum

Chun-Hat Shih1,2, Ivan K. Chu2,3, Wing Kin Yip1 and Clive Lo1,*

1 Department of Botany, The University of Hong Kong, Pokfulam Road, Hong Kong, PR China
2 Department of Chemistry, The University of Hong Kong, Pokfulam Road, Hong Kong, PR China
3 Genome Research Centre, The University of Hong Kong, Pokfulam Road, Hong Kong, PR China

* Corresponding author: E-mail, clivelo{at}hkucc.hku.hk; Fax, +852-2858-3477.

Three unique sorghum flavonoid 3'-hydroxylase (F3'H) cDNAs (SbF3'H1, SbF3'H2 and SbF3'H3) were discovered through bioinformatics analysis. Their encoded proteins showed >60% identity to the Arabidopsis TT7 (F3'H) protein. Overexpression of SbF3'H1 or SbF3'H2 restored the ability of tt7 mutants to produce 3'-hydroxylaed flavonoids, establishing their roles as functional F3'H enzymes. In sorghum mesocotyls, SbF3'H1 expression was involved in light-specific anthocyanin accumulation while SbF3'H2 expression was involved in pathogen-specific 3-deoxyanthocyanidin synthesis. No SbF3'H3 expression was detected in all tissues examined. The sorghum mesocotyls represent a good system for investigation of differential regulation of F3'H genes/alleles responding to different external stimuli.

The nucleotide sequences reported in this paper have been submitted to GenBank under accession numbers DQ787855–7 (SbF3'H1-SbF3'H3).

(Received August 1, 2006; Accepted August 16, 2006)
Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?




Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.