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Plant and Cell Physiology Advance Access originally published online on August 22, 2006
Plant and Cell Physiology 2006 47(10):1406-1411; doi:10.1093/pcp/pcl001
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© The Author 2006. Published by Oxford University Press on behalf of Japanese Society of Plant Physiologists. All rights reserved. For permissions, please email: journals.permissions@oxfordjournals.org

Short Communication

Arabidopsis Tubulin Folding Cofactor B Interacts with {alpha}-Tubulin In Vivo

Pankaj Dhonukshe1,3,*, Bastiaan O.R. Bargmann2,4 and Theodorus W.J. Gadella, Jr1,*

1 Section of Molecular Cytology, Swammerdam Institute for Life Sciences, University of Amsterdam, Kruislaan 316, 1098 SM Amsterdam, The Netherlands
2 Section of Plant Physiology, Swammerdam Institute for Life Sciences, University of Amsterdam, Kruislaan 318, 1098 SM Amsterdam, The Netherlands

* Corresponding authors: Pankaj Dhonukshe, E-mail, pankaj.dhonukshe{at}zmbp.uni-tuebingen.de; Fax, +49-7071-295797, Theodorus W.J. Gadella Jr, E-mail, gadella{at}science.uva.nl; Fax, +31-20-5256271.

Microtubule biogenesis requires {alpha}ß tubulin dimers that are generated from {alpha} and ß tubulin following post-translational modification by several tubulin folding cofactors (TFCs). Here we report the isolation and characterization of Arabidopsis TFCB (AtTFCB). AtTFCB is expressed in all organs of Arabidopsis. The subcellular localization of AtTFCB is mainly cytosolic. AtTFCB–overexpressing cells have fewer microtubules compared with the controls. Multimode fluorescence resonance energy transfer (FRET) microscopy reveals a direct physical interaction of AtTFCB with {alpha} tubulin in living plant cells. We conclude that AtTFCB interacts with {alpha} tubulin in vivo and its overexpression reduces the number of microtubules.

3 Present address: Center for Plant Molecular Biology (ZMBP), University of Tübingen, Auf der Morgenstelle, 72076 Tübingen, Germany.

4 Present address: New York University, Department of Biology, 100 Washington Square East, 1009 Silver Building, New York, NY 10003, USA.

(Received June 15, 2006; Accepted August 12, 2006)
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