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Plant and Cell Physiology Advance Access originally published online on July 16, 2005
Plant and Cell Physiology 2005 46(9):1525-1539; doi:10.1093/pcp/pci165
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The Effect of Overexpression of Two Brassica CBF/DREB1-like Transcription Factors on Photosynthetic Capacity and Freezing Tolerance in Brassica napus

Leonid V. Savitch1, Ghislaine Allard1, Motoaki Seki2, Laurian S. Robert1, Nicholas A. Tinker1, Norman P. A. Huner3, Kazuo Shinozaki2 and Jas Singh1,*

1 Eastern Cereal and Oilseed Research Centre, Agriculture and Agri-Food Canada, Central Experimental Farm, Ottawa, Ontario, Canada, K1A 0C6
2 Biological Resources Division, Japan International Research Centre for Agricultural Sciences, 1-1 Ohwashi, Tsukuba, Ibaraki, 305-8686 Japan
3 Department of Biology and the Biotron, University of Western Ontario, London, ON, Canada, N6A 5B7

* Corresponding author: Email, singhja{at}agr.gc.ca; Fax, 1-613-7591701.

The effects of overexpression of two Brassica CBF/DREB1-like transcription factors (BNCBF5 and 17) in Brassica napus cv. Westar were studied. In addition to developing constitutive freezing tolerance and constitutively accumulating COR gene mRNAs, BNCBF5- and 17-overexpressing plants also accumulate moderate transcript levels of genes involved in photosynthesis and chloroplast development as identified by microarray and Northern analyses. These include GLK1- and GLK2-like transcription factors involved in chloroplast photosynthetic development, chloroplast stroma cyclophilin ROC4 (AtCYP20–3), ß-amylase and triose-P/Pi translocator. In parallel with these changes, increases in photosynthetic efficiency and capacity, pigment pool sizes, increased capacities of the Calvin cycle enzymes, and enzymes of starch and sucrose biosynthesis, as well as glycolysis and oxaloacetate/malate exchange are seen, suggesting that BNCBF overexpression has partially mimicked cold-induced photosynthetic acclimation constitutively. Taken together, these results suggest that BNCBF/DREB1 overexpression in Brassica not only resulted in increased constitutive freezing tolerance but also partially regulated chloroplast development to increase photochemical efficiency and photosynthetic capacity.

(Received May 6, 2005; Accepted July 7, 2005)
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