Plant and Cell Physiology Advance Access originally published online on June 20, 2005
Plant and Cell Physiology 2005 46(9):1477-1483; doi:10.1093/pcp/pci159
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
The PsbH protein is associated with the inner antenna CP47 and facilitates D1 processing and incorporation into PSII in the cyanobacterium Synechocystis PCC 6803
1,2
1 Laboratory of Photosynthesis, Institute of Microbiology, Academy of Sciences, 379 81 Trebon, Czech Republic
2 Institute of Physical Biology, University of South Bohemia, 373 33 Nové Hrady, Czech Republic
3 The Institute of Botany, Ludwig-Maximilian University, Menzinger Str. 67, D-80368 Munich, Germany
* Corresponding author: E-mail, komenda{at}alga.cz; Fax, +420-384721246.
Analysis of a number of PSII complexes detectable in the wild-type and mutant cells of the cyanobacterium Synechocystis sp. PCC 6803 showed that the PsbH protein is present in the complexes containing CP47, including unassembled CP47. In a mutant lacking CP47, in which the PSII assembly is stopped at the level of the D1–D2–cytochrome b-559 reaction centre complex, a negligible amount of the PsbH protein was not bound to this complex but was detected in the free form. The results indicate that the PsbH protein has a high affinity for CP47 and during PSII assembly most probably first associates with CP47 and this pair is subsequently attached to the reaction centre complex. Similarly to CP47, the PsbH protein exhibits a slow light-induced degradation in the presence of protein synthesis inhibitor. The absence of the PsbH protein leads to a greatly increased D1 pool that is not associated with other PSII proteins or it is present as a part of the reaction centre complex. We conclude that PsbH is important for the prompt incorporation of the newly synthesized D1 protein into PSII complexes and for the fast D1 maturation.
(Received May 17, 2005; Accepted June 16, 2005)
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  M. Boehm, J. Nield, P. Zhang, E.-M. Aro, J. Komenda, and P. J. Nixon Structural and Mutational Analysis of Band 7 Proteins in the Cyanobacterium Synechocystis sp. Strain PCC 6803 J. Bacteriol., October 15, 2009; 191(20): 6425 - 6435. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Dobakova, R. Sobotka, M. Tichy, and J. Komenda Psb28 Protein Is Involved in the Biogenesis of the Photosystem II Inner Antenna CP47 (PsbB) in the Cyanobacterium Synechocystis sp. PCC 6803 Plant Physiology, February 1, 2009; 149(2): 1076 - 1086. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Schottkowski, S. Gkalympoudis, N. Tzekova, C. Stelljes, D. Schunemann, E. Ankele, and J. Nickelsen Interaction of the Periplasmic PratA Factor and the PsbA (D1) Protein during Biogenesis of Photosystem II in Synechocystis sp. PCC 6803 J. Biol. Chem., January 16, 2009; 284(3): 1813 - 1819. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Komenda, J. Nickelsen, M. Tichy, O. Prasil, L. A. Eichacker, and P. J. Nixon The Cyanobacterial Homologue of HCF136/YCF48 Is a Component of an Early Photosystem II Assembly Complex and Is Important for Both the Efficient Assembly and Repair of Photosystem II in Synechocystis sp. PCC 6803 J. Biol. Chem., August 15, 2008; 283(33): 22390 - 22399. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Dobakova, M. Tichy, and J. Komenda Role of the PsbI Protein in Photosystem II Assembly and Repair in the Cyanobacterium Synechocystis sp. PCC 6803 Plant Physiology, December 1, 2007; 145(4): 1681 - 1691. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Promnares, J. Komenda, L. Bumba, J. Nebesarova, F. Vacha, and M. Tichy Cyanobacterial Small Chlorophyll-binding Protein ScpD (HliB) Is Located on the Periphery of Photosystem II in the Vicinity of PsbH and CP47 Subunits J. Biol. Chem., October 27, 2006; 281(43): 32705 - 32713. [Abstract] [Full Text] [PDF]
|
|