Plant and Cell Physiology Advance Access originally published online on March 25, 2005
Plant and Cell Physiology 2005 46(5):782-789; doi:10.1093/pcp/pci085
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Peroxisomal Localization of a Myosin XI Isoform in Arabidopsis thaliana
1 Department of Life Science, Graduate School of Life Science, University of Hyogo, Harima Science Park City, Hyogo, 678-1297 Japan
2 Department of Cell Biology, National Institute for Basic Bology, Okazaki, 444-8585 Japan
3 Corresponding author: E-mail, 0510hk{at}sci.himeji-tech.ac.jp; Fax, +81-791-58-0175.
The genome of Arabidopsis thaliana contains 13 myosin XI isoforms. Here we prepared a specific antibody against a peptide that mimics a unique C-terminal region from the myosin XI isoform, MYA2. The resulting antibody was used to demonstrate that MYA2 in Arabidopsis protein extracts co-sedimented with actin filaments and dissociated from the filaments with ATP treatment. Immunolocalization studies showed that MYA2 co-localized predominantly with actin filaments in clustered punctuate dots in leaf epidermal cells, root hair cells and suspension-cultured cells. In a transgenic plant in which peroxisomes are labeled with green fluorescent protein, some MYA2 signals were localized on peroxisomes in an actin-dependent manner. We propose that the peroxisome is one of the cargos translocated by MYA2 on actin filaments.
(Received April 23, 2004; Accepted March 8, 2005
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