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Plant and Cell Physiology Advance Access originally published online on February 2, 2005
Plant and Cell Physiology 2005 46(3):482-488; doi:10.1093/pcp/pci046
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JSPP © 2005

Cellular Internalization of Fluorescent Proteins via Arginine-rich Intracellular Delivery Peptide in Plant Cells

Microsugar Chang1,2, Jyh-Ching Chou1 and Han-Jung Lee1,3

1 Institute of Biotechnology and Department of Life Science, National Dong Hwa University, Hualien 974, Taiwan
2 Institute of Medical Sciences, Tzu Chi University, Hualien 970, Taiwan

3 Corresponding author: E-mail, hjlee{at}mail.ndhu.edu.tw; Fax, +886-3-8630340.

The protein delivery across cellular membranes or compartments is limited by low biomembrane permeability because of the hydrophobic characteristics of cell membranes. Usually the delivery processes utilize passive protein channels or active transporters to overcome the membrane impediment. In this report, we demonstrate that arginine-rich intracellular delivery (AID) peptide is capable of efficiently delivering fused fluorescent proteins unpreferentially into different plant tissues of both tomato (a dicot plant) and onion (a monocot plant) in a fully bioactive form. Thus, cellular internalization via AID peptide can be a powerful tool characterized by its simplicity, non-invasion and high efficiency to express those bioactive proteins in planta or in plant cells in vivo. This novel method may alternatively provide broader applications of AID chimera in plants without the time-consuming transgenic approaches.

Received October 26, 2004; Accepted December 27, 2004
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