Plant and Cell Physiology Advance Access originally published online on August 24, 2005
Plant and Cell Physiology 2005 46(10):1724-1734; doi:10.1093/pcp/pci188
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Localization of NAD-Isocitrate Dehydrogenase and Glutamate Dehydrogenase in Rice Roots: Candidates for Providing Carbon Skeletons to NADH-Glutamate Synthase
1 Graduate School of Agricultural Science, Tohoku University, 1-1 Tsutsumidori-Amamiyamachi, Aoba-ku, Sendai, 981-8555 Japan
2 CREST, JST (Japan Science and Technology Agency), 4-1-8 Honcho, Kawaguchi, Saitama, 332-0012 Japan
3 Institut de Biotechnologie des Plantes (UMR8618 CNRS), Bâtiment 630, Université de Paris Sud-XI, 91405 Orsay cedex, France
* Corresponding author: E-mail, tyamaya{at}biochem.tohoku.ac.jp; Fax, +81-22-717-8787.
In rice roots, transient and cell-type-specific accumulation of both mRNA and protein for NADH-dependent glutamate synthase (NADH-GOGAT) occurs after the supply of NH4 + ions. In order to better understand the origin of 2-oxoglutarate for this reaction, we focused on mitochondrial NAD-dependent isocitrate dehydrogenase (IDH) and glutamate dehydrogenase (GDH) in rice roots. Six rice cDNAs encoding a single catalytic (OsIDHa) and two regulatory (OsIDHc;1, OsIDHc;2) IDH subunits and three GDH proteins (OsGDH1-3) were isolated. These genes, except OsGDH3, were expressed in the roots. Real-time PCR analysis showed that OsIDHa and OsIDHc;1 transcripts, but not OsGDH1 and OsGDH2 transcripts, accumulated in a similar manner to NADH-GOGAT mRNA along the crown roots after the supply of different forms of inorganic nitrogen. Furthermore, immunolocalization studies revealed the NH4 + induction of IDHa protein in two cell layers of the root surface, i.e. epidermis and exodermis, where NADH-GOGAT also accumulated. The possible relationship between NADH-GOGAT, IDH and GDH is discussed.
The nucleotide sequences reported in this paper have been submitted to GenBank/EMBL/DDBJ under the accession No. of AB024962 [GenBank] for OsGDH1 cDNA, AB189166 [GenBank] for OsGDH2 cDNA, AB035927 [GenBank] for OsGDH3 cDNA, AB189167 [GenBank] for OsIDHa cDNA, AB189168 [GenBank] for OsIDHc;1 cDNA and AB189169 [GenBank] for OsIDHc;2 cDNA, respectively.
(Received April 20, 2005; Accepted August 10, 2005)
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  M. Tabuchi, T. Abiko, and T. Yamaya Assimilation of ammonium ions and reutilization of nitrogen in rice (Oryza sativa L.) J. Exp. Bot., July 1, 2007; 58(9): 2319 - 2327. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Lemaitre, E. Urbanczyk-Wochniak, V. Flesch, E. Bismuth, A. R. Fernie, and M. Hodges NAD-Dependent Isocitrate Dehydrogenase Mutants of Arabidopsis Suggest the Enzyme Is Not Limiting for Nitrogen Assimilation Plant Physiology, July 1, 2007; 144(3): 1546 - 1558. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. S. Skopelitis, N. V. Paranychianakis, K. A. Paschalidis, E. D. Pliakonis, I. D. Delis, D. I. Yakoumakis, A. Kouvarakis, A. K. Papadakis, E. G. Stephanou, and K. A. Roubelakis-Angelakis Abiotic Stress Generates ROS That Signal Expression of Anionic Glutamate Dehydrogenases to Form Glutamate for Proline Synthesis in Tobacco and Grapevine PLANT CELL, October 1, 2006; 18(10): 2767 - 2781. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Lemaitre and M. Hodges Expression Analysis of Arabidopsis thaliana NAD-dependent Isocitrate Dehydrogenase Genes Shows the Presence of a Functional Subunit That Is Mainly Expressed in the Pollen and Absent from Vegetative Organs Plant Cell Physiol., May 1, 2006; 47(5): 634 - 643. [Abstract] [Full Text] [PDF]
|
|