Plant and Cell Physiology Advance Access originally published online on January 19, 2005
Plant and Cell Physiology 2005 46(1):224-232; doi:10.1093/pcp/pci017
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
© 2005 Oxford University Press
Involvement of Extracellular Dilignols in Lignification During Tracheary Element Differentiation of Isolated Zinnia Mesophyll Cells
1 Department of Biology and Earth Sciences, Faculty of Science, Ehime University, Matsuyama, 790-8577 Japan
2 Laboratory of Metabolic Science of Forest Plants and Microorganisms, Research Institute for Sustainable Humanosphere, Kyoto University, Uji, Kyoto, 611-0011 Japan
3 Department of Biological Sciences, Graduate School of Science, The University of Tokyo, Hongo, Bunkyo-ku, Tokyo, 113-0033 Japan
4 Plant Science Center, RIKEN, 1-7-22 Suehiro, Tsurumi-ku, Yokohama-shi, Kanagawa, 230-0045 Japan
During differentiation of isolated Zinnia mesophyll cells into tracheary elements (TEs), lignification on TEs progresses by supply of monolignols not only from TEs themselves but also from surrounding xylem parenchyma-like cells through the culture medium. However, how lignin polymerizes from the secreted monolignols has not been resolved. In this study, we analyzed phenol compounds in culture medium with reversed-phase HPLC, gas chromatography–mass spectrometry and nuclear magnetic resonance spectrometry, and found 12 phenolic compounds including coniferyl alcohol and four dilignols, i.e. erythro-guaiacylglycerol-ß-coniferyl ether, threo-guaiacylglycerol-ß-coniferyl ether, dehydrodiconiferyl alcohol and pinoresinol, in the medium in which TEs were developing. Coniferyl alcohol applied to TE-inductive cultures during TE formation rapidly disappeared from the medium, and caused a sudden increase in dilignols. Addition of the dilignols promoted lignification of TEs in which monolignol biosynthesis was blocked by an inhibitor of phenylalanine anmmonia-lyase (PAL), L-
-aminooxy-ß-phenylpropionic acid (AOPP). These results suggested that dilignols can act as intermediates of lignin polymerization.
5 Corresponding author: E-mail, ysato{at}sci.ehime-u.ac.jp; Fax, +81-89-927-9630.
(Received March 3, 2004; Accepted November 5, 2004)
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  Y. Sato, T. Demura, K. Yamawaki, Y. Inoue, S. Sato, M. Sugiyama, and H. Fukuda Isolation and Characterization of a Novel Peroxidase Gene ZPO-C Whose Expression and Function are Closely Associated with Lignification during Tracheary Element Differentiation Plant Cell Physiol., April 1, 2006; 47(4): 493 - 503. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. Ezaki, K. Sasaki, H. Matsumoto, and S. Nakashima Functions of two genes in aluminium (Al) stress resistance: repression of oxidative damage by the AtBCB gene and promotion of efflux of Al ions by the NtGDI1gene J. Exp. Bot., October 1, 2005; 56(420): 2661 - 2671. [Abstract] [Full Text] [PDF]
|
|