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Plant and Cell Physiology 2004 45(8):976-984; doi:10.1093/pcp/pch126
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© 2004 Oxford University Press

Enhanced Triterpene and Phytosterol Biosynthesis in Panax ginseng Overexpressing Squalene Synthase Gene

Mi-Hyun Lee1, Jae-Hun Jeong1, Jin-Wook Seo1, Cha-Gyun Shin1, Young-Soon Kim2, Jun-Gygo In3, Deok-Chun Yang3, Jae-Seon Yi4 and Yong-Eui Choi4,5

1 Korea Ginseng Institute, Chung-Ang University, Ansung 456-756, Kyunggi-do, Korea
2 Kumho life and Environmental Science Laboratory, Kumho Petrochemical Co., Kwangju 500-712, Korea
3 Department of Oriental Medical Materials and Processing, Kyung-Hee University, Suwon 449-701, Kyunggi-do, Korea
4 Division of Forest Resources, College of Forest Sciences, Kangwon National University, Chunchon, Kangwon-do, Korea

Roots of Panax ginseng, one of the most famous medicinal plants, contain various phytosterols and bioactive triterpene saponins (ginsenosides). In P. ginseng, phytosterols and triterpenes share the common biosynthetic intermediate, squalene. Here, we investigate the regulatory role of Panax ginseng squalene synthase (PgSS1) on the biosynthesis of phytosterols and triterpene saponins. PgSS1 transcripts are expressed ubiquitously in the various plant tissues, but higher in shoot apex and root. The transcript levels of PgSS1 increased markedly in the adventitious roots during 12- to 96-h period after metyl jasmonate (MeJA) treatment; MeJA treatment induced the activation of the transcripts of squalene epoxidase (SE), ß-amyrin synthase (bAS), but not cycloartenol synthase (CAS). Unlike MeJA treatment, overexpression of PgSS1 in adventitious roots of transgenic P. ginseng was followed by the up-regulation of all the downstream genes tested, such as SE, bAS, and CAS. The enhanced activity of PgSS1 enzyme resulted in remarkable increase of phytosterols as well as ginsenoside contents. These results demonstrate that PgSS1 is a key regulatory enzyme not only for phytosterol but also for triterpene biosynthesis and overexpressing of PgSS1 confers the hyperproduction of triterpene saponins to P. ginseng.

5 Corresponding author: E-mail, yechoi{at}kangwon.ac.kr; Fax, +82-33-252-8310.


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