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Plant and Cell Physiology 2004 45(7):951-957; doi:10.1093/pcp/pch105
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© 2004 Oxford University Press

Short Communication

Contribution of the Plasma Membrane and Central Vacuole in the Formation of Autolysosomes in Cultured Tobacco Cells

Kanako Yano1, Sumiko Matsui1, Tomohiro Tsuchiya2, Masayoshi Maeshima2, Natsumaro Kutsuna3, Seiichiro Hasezawa3 and Yuji Moriyasu1,4

1 School of Food and Nutritional Sciences, University of Shizuoka, Shizuoka, Japan
2 Graduate School of Bioagricultural Sciences, Nagoya University, Nagoya, Japan
3 Department of Integrated Biosciences, Graduate School of Frontier Sciences, University of Tokyo, Kashiwa, Japan

Abstract

Autolysosomes accumulate in tobacco cells cultured under sucrose starvation conditions in the presence of a cysteine protease inhibitor. We characterized these plant autolysosomes using fluorescent dyes and green fluorescent protein (GFP). Observation using the endocytosis markers, FM4-64 and Lucifer Yellow CH, suggested that there is a membrane flow from the plasma membrane to autolysosomes. Using these dyes as well as GFP-AtVam3p, sporamin-GFP and gamma-VM23-GFP fusion proteins as markers of the central vacuole, we found transport of components of the central vacuole to autolysosomes. Thus endocytosis and the supply from the central vacuole may contribute to the formation of autolysosomes.

Footnotes

4 Corresponding author: E-mail, moriyasu{at}u-shizuoka-ken.ac.jp; Fax, +81–54–264–5099.


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