Skip Navigation

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (20)
Right arrowRequest Permissions
Google Scholar
Right arrow Articles by Minoda, A.
Right arrow Articles by Tanaka, K.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Minoda, A.
Right arrow Articles by Tanaka, K.
Agricola
Right arrow Articles by Minoda, A.
Right arrow Articles by Tanaka, K.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Plant and Cell Physiology, 2004, Vol. 45, No. 6 667-671
© 2004 Oxford University Press

Rapid Paper

Improvement of Culture Conditions and Evidence for Nuclear Transformation by Homologous Recombination in a Red Alga, Cyanidioschyzon merolae 10D

Ayumi Minoda1, Rei Sakagami2, Fumi Yagisawa3,4, Tsuneyoshi Kuroiwa3 and Kan Tanaka1,5

1 Institute of Molecular and Cellular Biosciences, The University of Tokyo, 1-1-1 Yayoi, Tokyo, 113-0032 Japan
2 School of Life Science, Tokyo University of Pharmacy and Life Science, Hachioji, Tokyo, 192-0392 Japan
3 Department of Life Science, College of Science, Rikkyo (St. Paul’s) University, Nishiikebukuro, Tokyo, 171-8501 Japan
4 Department of Biological Sciences, Graduate School of Science, The University of Tokyo, Hongo, Tokyo, 113-0033 Japan

Although the nuclear genome sequence of Cyanidioschyzon merolae 10D, a unicellular red alga, was recently determined, DNA transformation technology that is important as a model plant system has never been available thus far. In this study, improved culture conditions resulted in a faster growth rate of C. merolae in liquid medium (doubling time = 9.2 h), and colony formation on gellan gum plates. Using these conditions, spontaneous mutants (5-fluoroortic acid resistant) deficient in the UMP synthase gene were isolated. The lesions were then restored by introducing the wild-type UMP synthase gene into the cells suggesting DNA transformation by homologous recombination.

5 Corresponding author: E-mail, kntanaka{at}iam.u-tokyo.ac.jp; Fax, +81-3-5841-8476.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Appl. Environ. Microbiol.Home page
J. A. Toplin, T. B. Norris, C. R. Lehr, T. R. McDermott, and R. W. Castenholz
Biogeographic and Phylogenetic Diversity of Thermoacidophilic Cyanidiales in Yellowstone National Park, Japan, and New Zealand
Appl. Envir. Microbiol., May 1, 2008; 74(9): 2822 - 2833.
[Abstract] [Full Text] [PDF]

Home page
 Plant Cell PhysiolHome page
M. Ohnuma, T. Yokoyama, T. Inouye, Y. Sekine, and K. Tanaka
Polyethylene Glycol (PEG)-Mediated Transient Gene Expression in a Red Alga, Cyanidioschyzon merolae 10D
Plant Cell Physiol., January 1, 2008; 49(1): 117 - 120.
[Abstract] [Full Text] [PDF]

Home page
 Plant Cell PhysiolHome page
T. Shimonaga, M. Konishi, Y. Oyama, S. Fujiwara, A. Satoh, N. Fujita, C. Colleoni, A. Buleon, J.-L. Putaux, S. G. Ball, et al.
Variation in Storage {alpha}-Glucans of the Porphyridiales (Rhodophyta)
Plant Cell Physiol., January 1, 2008; 49(1): 103 - 116.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Biol.Home page
M. S. Gentry, R. H. Dowen III, C. A. Worby, S. Mattoo, J. R. Ecker, and J. E. Dixon
The phosphatase laforin crosses evolutionary boundaries and links carbohydrate metabolism to neuronal disease
J. Cell Biol., July 24, 2007; 178(3): 477 - 488.
[Abstract] [Full Text] [PDF]

Home page
 Eukaryot CellHome page
F. X. Cunningham Jr., H. Lee, and E. Gantt
Carotenoid Biosynthesis in the Primitive Red Alga Cyanidioschyzon merolae
Eukaryot. Cell, March 1, 2007; 6(3): 533 - 545.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
S. G. Ball
Eukaryotic Microalgae Genomics. The Essence of Being a Plant
Plant Physiology, February 1, 2005; 137(2): 397 - 398.
[Full Text] [PDF]

Home page
 Plant Physiol.Home page
A. R. Grossman
Paths toward Algal Genomics
Plant Physiology, February 1, 2005; 137(2): 410 - 427.
[Full Text] [PDF]

Home page
 Plant Physiol.Home page
G. Barbier, C. Oesterhelt, M. D. Larson, R. G. Halgren, C. Wilkerson, R. M. Garavito, C. Benning, and A. P.M. Weber
Comparative Genomics of Two Closely Related Unicellular Thermo-Acidophilic Red Algae, Galdieria sulphuraria and Cyanidioschyzon merolae, Reveals the Molecular Basis of the Metabolic Flexibility of Galdieria sulphuraria and Significant Differences in Carbohydrate Metabolism of Both Algae
Plant Physiology, February 1, 2005; 137(2): 460 - 474.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
O. Misumi, M. Matsuzaki, H. Nozaki, S.-y. Miyagishima, T. Mori, K. Nishida, F. Yagisawa, Y. Yoshida, H. Kuroiwa, and T. Kuroiwa
Cyanidioschyzon merolae Genome. A Tool for Facilitating Comparable Studies on Organelle Biogenesis in Photosynthetic Eukaryotes
Plant Physiology, February 1, 2005; 137(2): 567 - 585.
[Abstract] [Full Text] [PDF]


Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.