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Plant and Cell Physiology, 2004, Vol. 45, No. 5 608-617
© 2004 Oxford University Press

Phosphorylation of Plasma Membrane Aquaporin Regulates Temperature-Dependent Opening of Tulip Petals

Abul Kalam Azad1,2, Yoshihiro Sawa1, Takahiro Ishikawa1 and Hitoshi Shibata1,3

1 Faculty of Life and Environmental Sciences, Shimane University, Matsue, Shimane, 690-8504 Japan
2 The United Graduate School of Agricultural Sciences, Tottori University, Koyama-Minami, Tottori, 680-0954 Japan

The opening and closing of tulip petals was reproduced in the dark by changing the temperature from 5°C to 20°C for opening and 20°C to 5°C for closing. The opening process was accompanied by 3H2O transport through the stem from the incubation medium to the petals. A Ca2+-channel blocker and a Ca2+-chelator inhibited petal opening and 3H2O transport. Several proteins in the isolated plasma membrane fraction were phosphorylated in the presence of 25 µM Ca2+ at 20°C. The 31-kDa protein that was phosphorylated, was suggested immunologically as the putative plasma membrane aquaporin (PM-AQP). This phosphorylated PM-AQP clearly reacted with the anti-phospho-Ser. In-gel assay revealed the presence of a 45-kDa Ca2+-dependent protein kinase in the isolated plasma membrane. Phosphorylation of the putative PM-AQP was thought to activate the water channel composed of PM-AQP. Dephosphorylation of the phosphorylated PM-AQP was also observed during petal closing at 5°C, suggesting the inactivation of the water channel.

3 Corresponding author; E-mail shibata{at}life.shimane-u.ac.jp


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