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Plant and Cell Physiology, 2004, Vol. 45, No. 4 496-500
© 2004 Oxford University Press

Short Communication

Functional Analysis of a Rice Putative Voltage-Dependent Ca2+ Channel, OsTPC1, Expressed in Yeast Cells Lacking its Homologous Gene CCH1

Kenji Hashimoto1, Mikako Saito1,4, Hideaki Matsuoka1,4,5, Kazuko Iida2 and Hidetoshi Iida3,4

1 Department of Biotechnology and Life Science, Tokyo University of Agriculture and Technology, Koganei, Tokyo, 184-8588 Japan
2 Medical R & D Center, The Tokyo Metropolitan Institute of Medical Science, Honkomagome 3-18-22, Bunkyo-ku, Tokyo, 113-8613 Japan
3 Department of Biology, Tokyo Gakugei University, Tokyo, 184-8501 Japan
4 CREST, Japan Science and Technology Agency, Saitama, 332-0012 Japan

Abstract

We isolated a cDNA (OsTPC1) from rice that was homologous to AtTPC1, a putative voltage-dependent Ca2+ channel (VDCC) gene of Arabidopsis thaliana. The hydropathy profile of its deduced amino acid sequence showed significant structural features of the {alpha}1-subunit of animal VDCCs. Functional analysis using a heterologous yeast expression system showed that OsTPC1 facilitates Ca2+ permeation. The Km value for Ca2+ of OsTPC1, 47.5 µM, was comparable to that of intrinsic CCH1, a candidate VDCC in yeast. Ca2+ permeation by OsTPC1 was inhibited by verapamil, a VDCC blocker. These findings indicate for the first time that OsTPC1 is a putative VDCC in rice.

Footnotes

5 Corresponding author: E-mail, bio-func{at}cc.tuat.ac.jp; Fax, +81-42–387-1503.


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