Skip Navigation

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (17)
Right arrowRequest Permissions
Google Scholar
Right arrow Articles by Henmi, T.
Right arrow Articles by Yamamoto, Y.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Henmi, T.
Right arrow Articles by Yamamoto, Y.
Agricola
Right arrow Articles by Henmi, T.
Right arrow Articles by Yamamoto, Y.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Plant and Cell Physiology, 2004, Vol. 45, No. 2 243-250
© 2004 Oxford University Press

Short Communication

Release and Reactive-Oxygen-Mediated Damage of the Oxygen-Evolving Complex Subunits of PSII during Photoinhibition

Takahiro Henmi1, Mitsue Miyao2 and Yasusi Yamamoto1,3

1 Graduate School of Natural Science and Technology, Okayama University, Okayama, 700-8530 Japan
2 Photosynthesis Laboratory, National Institute of Agrobiological Sciences (NIAS), Kannondai, Tsukuba, 305-8602 Japan

Abstract

Under photoinhibitory illumination of spinach PSII membranes, the oxygen-evolving complex subunits, OEC33, 24 and 18, were released from PSII. The liberated OEC33 and also OEC24 to a lesser extent were subsequently damaged and then exhibited smeared bands in SDS/urea-PAGE. Once deteriorated, OEC33 could not bind to PSII. The effects of scavengers and chelating reagents on the damage indicated that hydroxyl radicals generated from superoxide in the presence of metal ions were responsible for the damage. These results suggest that, like the D1 protein of the PSII reaction center complex, OEC subunits suffer oxidative damage and turnover under illumination.

Footnotes

3 Corresponding author: E-mail, yasusiya{at}cc.okayama-u.ac.jp; Fax, +81-86-251-7876.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 J. Biol. Chem.Home page
A. Yamashita, N. Nijo, P. Pospisil, N. Morita, D. Takenaka, R. Aminaka, Y. Yamamoto, and Y. Yamamoto
Quality Control of Photosystem II: REACTIVE OXYGEN SPECIES ARE RESPONSIBLE FOR THE DAMAGE TO PHOTOSYSTEM II UNDER MODERATE HEAT STRESS
J. Biol. Chem., October 17, 2008; 283(42): 28380 - 28391.
[Abstract] [Full Text] [PDF]

Home page
 Plant Cell PhysiolHome page
W. Zhao, Q. Guo, and J. Zhao
A Membrane-Associated Mn-Superoxide Dismutase Protects the Photosynthetic Apparatus and Nitrogenase from Oxidative Damage in the Cyanobacterium Anabaena sp. PCC 7120
Plant Cell Physiol., April 1, 2007; 48(4): 563 - 572.
[Abstract] [Full Text] [PDF]

Home page
 Plant Cell PhysiolHome page
D. Kornyeyev, B. A. Logan, D. T. Tissue, R. D. Allen, and A. S. Holaday
Compensation for PSII Photoinactivation by Regulated Non-photochemical Dissipation Influences the Impact of Photoinactivation on Electron Transport and CO2 Assimilation
Plant Cell Physiol., April 1, 2006; 47(4): 437 - 446.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.