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Plant and Cell Physiology 2004 45(12):1882-1888; doi:10.1093/pcp/pch211
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© 2004 Oxford University Press

Short Communication

Identification and Subcellular Localization of Two Solanesyl Diphosphate Synthases from Arabidopsis thaliana

Luo Jun1, Ryoichi Saiki1, Kei Tatsumi1, Tsuyoshi Nakagawa2 and Makoto Kawamukai1,3

1 Department of Applied Bioscience and Biotechnology, Faculty of Life and Environmental Science, Shimane University, 1060 Nishikawatsu, Matsue, 690-8504 Japan
2 Center for Integrated Research in Science, Shimane University, Matsue, 690-8504 Japan

Two solanesyl diphosphate synthases, designated SPS1 and SPS2, which are responsible for the synthesis of the isoprenoid side chain of either plastoquinone or ubiquinone in Arabidopsis thaliana, were identified. Heterologous expression of either SPS1 or SPS2 allowed the generation of UQ-9 in a decaprenyl diphosphate synthase-defective strain of fission yeast and also in wild-type Escherichia coli. SPS1–GFP was found to localize in the ER while SPS2–GFP localized in the plastid of tobacco BY-2 cells. These two different subcellular localizations are thought to be the reflection of their roles in solanesyl diphosphate synthesis in two different parts: presumably SPS1 and SPS2 for the side chains of ubiquinone and plastoquinone, respectively.

3 Corresponding author: E-mail, kawamuka{at}life.shimane-u.ac.jp; Fax, +81-852-32-6092.


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