Skip Navigation

Plant and Cell Physiology 2004 45(11):1658-1668; doi:10.1093/pcp/pch188
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrowRequest Permissions
Google Scholar
Right arrow Articles by Takahata, K.
Right arrow Articles by Sato, F.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Takahata, K.
Right arrow Articles by Sato, F.
Agricola
Right arrow Articles by Takahata, K.
Right arrow Articles by Sato, F.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

© 2004 Oxford University Press

Isolation of Putative Glycoprotein Gene from Early Somatic Embryo of Carrot and its Possible Involvement in Somatic Embryo Development

Kiminori Takahata1, Miyuki Takeuchi2, Minoru Fujita2, Junichi Azuma3, Hiroshi Kamada4 and Fumihiko Sato1,5

1 Department of Plant Gene and Totipotency, Division of Integrated Life Science, Graduate School of Biostudies, Kyoto University, Sakyo, Kyoto, 606-8502 Japan
2 Division of Forest Biomaterials Science, Graduate School of Agriculture, Kyoto University, Sakyo, Kyoto, 606-8502 Japan
3 Division of Environmental Science and Technology, Graduate School of Agriculture, Kyoto University, Sakyo, Kyoto, 606-8502 Japan
4 Institute of Biological Sciences, University of Tsukuba, Tsukuba, Ibaraki, 305-8572 Japan

Somatic embryogenesis is a unique process in plant cells. For example, embryogenic cells (EC) of carrot (Daucus carota) maintained in a medium containing 2,4-dichlorophenoxyacetic acid (2,4-D) regenerate whole plants via somatic embryogenesis after the depletion of 2,4-D. Although some genes such as C-ABI3 and C-LEC1 have been found to be involved in somatic embryogenesis, the critical molecular and cellular mechanisms for somatic embryogenesis are unknown. To characterize the early mechanism in the induction of somatic embryogenesis, we isolated genes expressed during the early stage of somatic embryogenesis after 2,4-D depletion. Subtractive hybridization screening and subsequent RNA gel blot analysis suggested a candidate gene, Carrot Early Somatic Embryogenesis 1 (C-ESE1). C-ESE1 encodes a protein that has agglutinin and S-locus-glycoprotein domains and its expression is highly specific to primordial cells of somatic embryo. Transgenic carrot cells with reduced expression of C-ESE1 had wide intercellular space and decreased polysaccharides on the cell surface and showed delayed development in somatic embryogenesis. The importance of cell-to-cell attachment in somatic embryogenesis is discussed.

5 Corresponding author: E-mail, fumihiko{at}kais.kyoto-u.ac.jp; Fax, +81-75-753-6398.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Plant Physiol.Home page
A. Siedlecka, S. Wiklund, M.-A. Peronne, F. Micheli, J. Lesniewska, I. Sethson, U. Edlund, L. Richard, B. Sundberg, and E. J. Mellerowicz
Pectin Methyl Esterase Inhibits Intrusive and Symplastic Cell Growth in Developing Wood Cells of Populus
Plant Physiology, February 1, 2008; 146(2): 554 - 565.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.