Skip Navigation

Plant and Cell Physiology 2004 45(11):1586-1594; doi:10.1093/pcp/pch181
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrowRequest Permissions
Google Scholar
Right arrow Articles by Yabuta, Y.
Right arrow Articles by Shigeoka, S.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Yabuta, Y.
Right arrow Articles by Shigeoka, S.
Agricola
Right arrow Articles by Yabuta, Y.
Right arrow Articles by Shigeoka, S.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

© 2004 Oxford University Press

Two Distinct Redox Signaling Pathways for Cytosolic APX Induction under Photooxidative Stress

Yukinori Yabuta1, Takanori Maruta1, Kazuya Yoshimura1, Takahiro Ishikawa2 and Shigeru Shigeoka1,3

1 Department of Food and Nutrition, Faculty of Agriculture, Kinki University, 3327-204 Nakamachi, Nara, 631-8505 Japan
2 Faculty of Life and Environmental Science, Shimane University, 1060 Nishikawatsu, Matsue, Shimane, 690-8504 Japan

The cross-talk of two redox factors, H2O2 accumulation and redox status of photosynthetic electron transport (PET) in tobacco chloroplasts, on the expression of cytosolic ascorbate peroxidase (cAPX) was studied. Transgenic tobacco plants, which expressed respectively the Escherichia coli catalase and spinach thylakoid-membrane-bound APX in chloroplasts and showed increased tolerance to photooxidative stress, were used for evaluation of the relationship between H2O2 accumulation or change of redox status of PET and cAPX induction under photooxidative stress condition. There was no difference in the increase in the transcript level of cAPX in the first 1 h after irradiation with high-intensity light between the wild-type and either of the transgenic plants. The transcript level of cAPX in the wild-type showed a steady gradual increase during the next 5 h, while that in the transgenic plants during this period was stable. The H2O2 level of wild-type plants increased after 1 h, while those of transgenic plants remained constant. The decrease in qp value in all types of plants occurred earlier than the increase in H2O2 level. These results indicate that the induction of cAPX expression is caused by a redox change in PET, probably through the plastquinone pool at an early stage and thereafter by an increase in the cellular H2O2 level.

3 Corresponding author: E-mail, shigeoka{at}nara.kindai.ac.jp; Fax, +81-742-43-2252.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Plant CellHome page
G. Galvez-Valdivieso, M. J. Fryer, T. Lawson, K. Slattery, W. Truman, N. Smirnoff, T. Asami, W. J. Davies, A. M. Jones, N. R. Baker, et al.
The High Light Response in Arabidopsis Involves ABA Signaling between Vascular and Bundle Sheath Cells
PLANT CELL, July 1, 2009; 21(7): 2143 - 2162.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
Y. Yabuta, T. Mieda, M. Rapolu, A. Nakamura, T. Motoki, T. Maruta, K. Yoshimura, T. Ishikawa, and S. Shigeoka
Light regulation of ascorbate biosynthesis is dependent on the photosynthetic electron transport chain but independent of sugars in Arabidopsis
J. Exp. Bot., August 11, 2007; (2007) erm124v3.
[Abstract] [Full Text] [PDF]

Home page
 Plant Cell PhysiolHome page
T. Ishikawa, Y. Morimoto, R. Madhusudhan, Y. Sawa, H. Shibata, Y. Yabuta, A. Nishizawa, and S. Shigeoka
Acclimation to Diverse Environmental Stresses Caused by a Suppression of Cytosolic Ascorbate Peroxidase in Tobacco BY-2 cells
Plant Cell Physiol., August 1, 2005; 46(8): 1264 - 1271.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
M. Baier and K.-J. Dietz
Chloroplasts as source and target of cellular redox regulation: a discussion on chloroplast redox signals in the context of plant physiology
J. Exp. Bot., June 1, 2005; 56(416): 1449 - 1462.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.