Plant and Cell Physiology, 2003, Vol. 44, No. 8 868-874
© 2003 Oxford University Press
Short Communications |
The Type-A Response Regulator, ARR15, Acts as a Negative Regulator in the Cytokinin-Mediated Signal Transduction in Arabidopsis thaliana
1 Laboratory of Molecular Microbiology, School of Agriculture, Nagoya University, Chikusa-ku, Nagoya 464-8601, Japan
2 Kazusa DNA Research Institute, Kazusa, Chiba 292-0812, Japan
Abstract
The Arabidopsis thaliana AHK4 histidine kinase (also known as CRE1 or WOL) acts as a cytokinin signal transducer, presumably, in concert with downstream components, such as histidine-containing phosphotransfer factors (AHPs) and response regulators (ARRs), through the histidine-to-aspartate (His
Asp) phosphorelay. Among 10 members of the type-A ARR family, the cytokinin-induced expression of ARR15 in roots is selectively impaired in the cre1-1 mutant, which carries a mutation in the AHK4 gene, suggesting a link between this type-A response regulator and the AHK4-mediated cytokinin signal transduction in roots. To address this issue further, we characterized a T-DNA insertion mutant of ARR15, and also constructed transgenic lines (referred to as ARR15-ox) that overexpress the ARR15 gene in a manner independent of cytokinin. While the T-DNA insertion mutant (arr15-1) showed no apparent phenotype, the cytokinin-independent overexpression of ARR15 in ARR15-ox plants resulted in a reduced sensitivity toward exogenously applied cytokinin, not only in elongation of roots in plants, but also in green callus formation (or shoot formation) in explants. Cytokinin-induced expressions of certain type-A ARRs were also down-regulated in ARR15-ox plants. These results support the view that ARR15 acts as a repressor that mediates a negative feedback loop in the cytokinin and AHK4-mediated HisAsp phosphorelay.
Footnotes
3 Corresponding author: E-mail, tmizuno{at}agr.nagoya-u.ac.jp; Fax, +81-52-789-4091.
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  K. Ishida, Y. Niwa, T. Yamashino, and T. Mizuno A Genome-Wide Compilation of the Two-Component Systems in Lotus japonicus DNA Res, August 1, 2009; 16(4): 237 - 247. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 F. Chevalier, D. Perazza, F. Laporte, G. Le Henanff, P. Hornitschek, J.-M. Bonneville, M. Herzog, and G. Vachon GeBP and GeBP-Like Proteins Are Noncanonical Leucine-Zipper Transcription Factors That Regulate Cytokinin Response in Arabidopsis Plant Physiology, March 1, 2008; 146(3): 1142 - 1154. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. Hirose, K. Takei, T. Kuroha, T. Kamada-Nobusada, H. Hayashi, and H. Sakakibara Regulation of cytokinin biosynthesis, compartmentalization and translocation J. Exp. Bot., January 1, 2008; 59(1): 75 - 83. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Ishida, T. Yamashino, A. Yokoyama, and T. Mizuno Three Type-B Response Regulators, ARR1, ARR10 and ARR12, Play Essential but Redundant Roles in Cytokinin Signal Transduction Throughout the Life Cycle of Arabidopsis thaliana Plant Cell Physiol., January 1, 2008; 49(1): 47 - 57. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. U. Andersen, S. Buechel, Z. Zhao, K. Ljung, O. Novak, W. Busch, C. Schuster, and J. U. Lohmann Requirement of B2-Type Cyclin-Dependent Kinases for Meristem Integrity in Arabidopsis thaliana PLANT CELL, January 1, 2008; 20(1): 88 - 100. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. P.C. To, J. Deruere, B. B. Maxwell, V. F. Morris, C. E. Hutchison, F. J. Ferreira, G. E. Schaller, and J. J. Kieber Cytokinin Regulates Type-A Arabidopsis Response Regulator Activity and Protein Stability via Two-Component Phosphorelay PLANT CELL, December 1, 2007; 19(12): 3901 - 3914. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Hirose, N. Makita, M. Kojima, T. Kamada-Nobusada, and H. Sakakibara Overexpression of a Type-A Response Regulator Alters Rice Morphology and Cytokinin Metabolism Plant Cell Physiol., March 1, 2007; 48(3): 523 - 539. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Taniguchi, N. Sasaki, T. Tsuge, T. Aoyama, and A. Oka ARR1 Directly Activates Cytokinin Response Genes that Encode Proteins with Diverse Regulatory Functions Plant Cell Physiol., February 1, 2007; 48(2): 263 - 277. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. P. Mane, C. Vasquez-Robinet, A. A. Sioson, L. S. Heath, and R. Grene Early PLD{alpha}-mediated events in response to progressive drought stress in Arabidopsis: a transcriptome analysis J. Exp. Bot., January 1, 2007; 58(2): 241 - 252. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Yokoyama, T. Yamashino, Y.-I. Amano, Y. Tajima, A. Imamura, H. Sakakibara, and T. Mizuno Type-B ARR Transcription Factors, ARR10 and ARR12, are Implicated in Cytokinin-Mediated Regulation of Protoxylem Differentiation in Roots of Arabidopsis thaliana Plant Cell Physiol., January 1, 2007; 48(1): 84 - 96. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. A. Salome, J. P.C. To, J. J. Kieber, and C. R. McClung Arabidopsis Response Regulators ARR3 and ARR4 Play Cytokinin-Independent Roles in the Control of Circadian Period PLANT CELL, January 1, 2006; 18(1): 55 - 69. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Kiba, T. Naitou, N. Koizumi, T. Yamashino, H. Sakakibara, and T. Mizuno Combinatorial Microarray Analysis Revealing Arabidopsis Genes Implicated in Cytokinin Responses through the His->Asp Phosphorelay Circuitry Plant Cell Physiol., February 1, 2005; 46(2): 339 - 355. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Kiba, K. Aoki, H. Sakakibara, and T. Mizuno Arabidopsis Response Regulator, ARR22, Ectopic Expression of Which Results in Phenotypes Similar to the wol Cytokinin-Receptor Mutant Plant Cell Physiol., August 15, 2004; 45(8): 1063 - 1077. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. P.C. To, G. Haberer, F. J. Ferreira, J. Deruere, M. G. Mason, G. E. Schaller, J. M. Alonso, J. R. Ecker, and J. J. Kieber Type-A Arabidopsis Response Regulators Are Partially Redundant Negative Regulators of Cytokinin Signaling PLANT CELL, March 1, 2004; 16(3): 658 - 671. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Tajima, A. Imamura, T. Kiba, Y. Amano, T. Yamashino, and T. Mizuno Comparative Studies on the Type-B Response Regulators Revealing their Distinctive Properties in the His-to-Asp Phosphorelay Signal Transduction of Arabidopsis thaliana Plant Cell Physiol., January 15, 2004; 45(1): 28 - 39. [Abstract] [Full Text] [PDF]
|
|