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Plant and Cell Physiology, 2003, Vol. 44, No. 7 776-781
© 2003 Oxford University Press

Short Communications

Effects of Antibiotics that Inhibit the Bacterial Peptidoglycan Synthesis Pathway on Moss Chloroplast Division

Nami Katayama1, Hiroyoshi Takano1,7, Motoji Sugiyama1, Susumu Takio2, Atsushi Sakai3, Kan Tanaka4, Haruko Kuroiwa5 and Kanji Ono6

1 Graduate School of Science and Technology, Kumamoto University, Kumamoto, 860-8555 Japan
2 Center for Marine Environment Studies, Kumamoto University, Kumamoto, 860-8555 Japan
3 Department of Biological Science, Faculty of Science, Nara Women’s University, Nara, 630-8506 Japan
4 Institute of Molecular and Cellular Biosciences, University of Tokyo, Tokyo, 113-0032 Japan
5 Department of Biological Sciences, Graduate School of Science, University of Tokyo, Tokyo, 113-0033 Japan
6 Department of Biological Science, Faculty of Science, Kumamoto University, Kumamoto, 860-8555 Japan

Abstract

Moss chloroplasts should prove useful for studying the cyanobacteria-derived system in chloroplasts. To determine the effects of antibiotics that inhibit bacterial peptidoglycan synthesis, the numbers of chloroplasts in treated Physcomitrella patens cells were counted. Ampicillin and D-cycloserine caused a rapid decrease in the number of chloroplasts per cell. Fosfomycin affected half of the cells, while vancomycin affected a few cells. Conversely, bacitracin had no effect. With the decrease in chloroplast number, macrochloroplasts appeared in antibiotic-treated cells. Removal of the antibiotics resulted in the recovery of chloroplast number, suggesting that the decrease in number was directly dependent on the antibiotic treatment. Microscopic observations showed that the decrease in the number of chloroplasts resulted from cell division without chloroplast division. These results suggest that enzymes derived from the bacterial peptidoglycan synthesis pathway are related to moss chloroplast division.

Footnotes

7 Corresponding author: E-mail, takano{at}kumamoto-u.ac.jp; Fax, +81-96-342-3432.


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 Proc. Natl. Acad. Sci. USAHome page
M. Machida, K. Takechi, H. Sato, S. J. Chung, H. Kuroiwa, S. Takio, M. Seki, K. Shinozaki, T. Fujita, M. Hasebe, et al.
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