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Plant and Cell Physiology, 2003, Vol. 44, No. 2 201-205
© 2003 Oxford University Press

Short Communications

Susceptibility of Chara Myosin to SH Reagents

Masaya Seki1, Jun-ya Awata1, Kiyo Shimada1, Taku Kashiyama2, Kohji Ito1 and Keiichi Yamamoto1,3

1 Department of Biology, Chiba University, 1-33 Yayoi-cho, Inage-ku, Chiba, 263-8522 Japan
2 Department of Pharmacology, Juntendo University School of Medicine, 2-1-1 Hongo, Tokyo, 113-8421 Japan

Abstract

Cellular and intracellular motile events in plants are susceptible to SH reagents such as N-ethylmaleimide (NEM). It has long been believed that the target of the reagent is myosin. We compared the effect of NEM on the motile and ATPase activities of skeletal muscle myosin with that on plant myosin using characean algal myosin. It was found that the motile activity of myosin prepared from NEM-treated C. corallina decreased to a level accountable for the decrease in the velocity of cytoplasmic streaming but it was also found that Chara myosin was far less susceptible to NEM than skeletal muscle myosin.

Footnotes

3 Corresponding author: E-mail: yamamoto{at}bio.s.chiba-u.ac.jp; Fax: +81-43-290-2809.


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