Plant and Cell Physiology, 2003, Vol. 44, No. 10 1045-1054
© 2003 Oxford University Press
Three-Dimensional Reconstruction of Tubular Structure of Vacuolar Membrane Throughout Mitosis in Living Tobacco Cells
1 Graduate School of Frontier Sciences, The University of Tokyo, Kashiwa, Chiba, 277-8562 Japan
2 Graduate School of Human and Environmental Studies, Kyoto University, Sakyo-ku, Kyoto, 606-8501 Japan
Plant vacuoles are the largest of organelles, performing various functions in cellular metabolism, morphogenesis and cell division. Dynamic changes in vacuoles during mitosis were studied by monitoring tubular structure of vacuolar membrane (TVM) in living transgenic tobacco BY-2 cells stably expressing a GFP-AtVam3p fusion protein (BY-GV). Comprehensive images of the complicated TVM configurations were obtained by reconstructing three-dimensional (3-D) surface structures from sequential confocal sections, using newly developed software, SSR (stereo-structure reconstructor). Using the surface modeling technique, we succeeded for the first time in clarifying the development process of TVMs and the topological relationship between TVMs and large vacuoles. TVMs, initially organized from large vacuoles, elongated to encircle the spindle at metaphase. Subsequently, the TVMs invaded the equatorial region from anaphase to telophase, and then they were divided to the two daughter cells by the cell plate at cytokinesis. When the daughter nuclei were separating from the cell plate, some TVMs enlarged to form large vacuoles near the division site. Spatial analysis revealed that from anaphase until cytokinesis, TVMs connected the two large vacuoles and functioned as a route for inter-vacuolar transport. Furthermore, the experiments using the inhibitor for actin microfilaments indicated that the microfilaments were indispensable for the development and the maintenance of TVMs.
3 Corresponding author: E-mail, hasezawa{at}k.u-tokyo.ac.jp; Fax, +81-4-7136-3706.
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  E. J. Wiltshire and D. A. Collings New Dynamics in an Old Friend: Dynamic Tubular Vacuoles Radiate Through the Cortical Cytoplasm of Red Onion Epidermal Cells Plant Cell Physiol., October 1, 2009; 50(10): 1826 - 1839. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Oda, A. Hirata, T. Sano, T. Fujita, Y. Hiwatashi, Y. Sato, A. Kadota, M. Hasebe, and S. Hasezawa Microtubules Regulate Dynamic Organization of Vacuoles in Physcomitrella patens Plant Cell Physiol., April 1, 2009; 50(4): 855 - 868. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. Okubo-Kurihara, T. Sano, T. Higaki, N. Kutsuna, and S. Hasezawa Acceleration of Vacuolar Regeneration and Cell Growth by Overexpression of an Aquaporin NtTIP1;1 in Tobacco BY-2 Cells Plant Cell Physiol., January 1, 2009; 50(1): 151 - 160. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Andeme Ondzighi, D. A. Christopher, E. J. Cho, S.-C. Chang, and L. A. Staehelin Arabidopsis Protein Disulfide Isomerase-5 Inhibits Cysteine Proteases during Trafficking to Vacuoles before Programmed Cell Death of the Endothelium in Developing Seeds PLANT CELL, August 1, 2008; 20(8): 2205 - 2220. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Higaki, T. Goh, T. Hayashi, N. Kutsuna, Y. Kadota, S. Hasezawa, T. Sano, and K. Kuchitsu Elicitor-Induced Cytoskeletal Rearrangement Relates to Vacuolar Dynamics and Execution of Cell Death: In Vivo Imaging of Hypersensitive Cell Death in Tobacco BY-2 Cells Plant Cell Physiol., October 1, 2007; 48(10): 1414 - 1425. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Tanaka, N. Kutsuna, Y. Kanazawa, N. Kondo, S. Hasezawa, and T. Sano Intra-Vacuolar Reserves of Membranes During Stomatal Closure: The Possible Role of Guard Cell Vacuoles Estimated by 3-D Reconstruction Plant Cell Physiol., August 1, 2007; 48(8): 1159 - 1169. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Iwano, H. Shiba, K. Matoba, T. Miwa, M. Funato, T. Entani, P. Nakayama, H. Shimosato, A. Takaoka, A. Isogai, et al. Actin Dynamics in Papilla Cells of Brassica rapa during Self- and Cross-Pollination Plant Physiology, May 1, 2007; 144(1): 72 - 81. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. J. Suh, Y.-F. Wang, A. Frelet, N. Leonhardt, M. Klein, C. Forestier, B. Mueller-Roeber, M. H. Cho, E. Martinoia, and J. I. Schroeder The ATP Binding Cassette Transporter AtMRP5 Modulates Anion and Calcium Channel Activities in Arabidopsis Guard Cells J. Biol. Chem., January 19, 2007; 282(3): 1916 - 1924. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. R. Darrah, M. Tlalka, A. Ashford, S. C. Watkinson, and M. D. Fricker The vacuole system is a significant intracellular pathway for longitudinal solute transport in basidiomycete fungi. Eukaryot. Cell, July 1, 2006; 5(7): 1111 - 1125. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Higaki, N. Kutsuna, E. Okubo, T. Sano, and S. Hasezawa Actin Microfilaments Regulate Vacuolar Structures and Dynamics: Dual Observation of Actin Microfilaments and Vacuolar Membrane in Living Tobacco BY-2 Cells Plant Cell Physiol., July 1, 2006; 47(7): 839 - 852. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J.-y. Shoji, M. Arioka, and K. Kitamoto Vacuolar Membrane Dynamics in the Filamentous Fungus Aspergillus oryzae Eukaryot. Cell, February 1, 2006; 5(2): 411 - 421. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
X.-Q. Gao, C.-G. Li, P.-C. Wei, X.-Y. Zhang, J. Chen, and X.-C. Wang The Dynamic Changes of Tonoplasts in Guard Cells Are Important for Stomatal Movement in Vicia faba Plant Physiology, November 1, 2005; 139(3): 1207 - 1216. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Yano, S. Matsui, T. Tsuchiya, M. Maeshima, N. Kutsuna, S. Hasezawa, and Y. Moriyasu Contribution of the Plasma Membrane and Central Vacuole in the Formation of Autolysosomes in Cultured Tobacco Cells Plant Cell Physiol., July 15, 2004; 45(7): 951 - 957. [Abstract] [Full Text] [PDF]
|
|