Skip Navigation

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (11)
Right arrowRequest Permissions
Google Scholar
Right arrow Articles by Okazaki, Y.
Right arrow Articles by Iwasaki, N.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Okazaki, Y.
Right arrow Articles by Iwasaki, N.
Agricola
Right arrow Articles by Okazaki, Y.
Right arrow Articles by Iwasaki, N.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Plant and Cell Physiology, 2002, Vol. 43, No. 9 1027-1035
© 2002 Oxford University Press

Temporal Relationship between Cytosolic Free Ca2+ and Membrane Potential during Hypotonic Turgor Regulation in a Brackish Water Charophyte Lamprothamnium succinctum

Yoshiji Okazaki1,3, Mitsuo Ishigami2 and Naohiko Iwasaki1

1 Department of Biology, Osaka Medical College, Takatsuki, Osaka, 569-8686 Japan
2 Department of Biology, Faculty of Education, Shiga University, Otsu, Shiga, 520-0862 Japan

Internodal cells of a brackish water charophyte, Lamprothamnium succinctum, regulate turgor pressure in response to changes in external osmotic pressure by modifying vacuolar concentrations of KCl. An increase in cytosolic concentration of free Ca2+ ([Ca2+]c) is necessary for the progress of turgor regulation induced by hypotonic treatment. Initial changes in membrane potential and [Ca2+]c upon hypotonic treatment were measured to examine the temporal relationship between the two parameters. Fura-dextran (potassium salt, Mr 10,000, anionic) that had been injected into the cytosol was used to measure [Ca2+]c. Membrane potential and membrane conductance under a current-clamp condition were also measured. Decrease in external osmotic pressure by 0.16 Osm induced a simultaneous increase in [Ca2+]c with both depolarization of the membrane and increase in the membrane conductance. Decrease in external osmotic pressure by 0.05 Osm induced a simultaneous increase in [Ca2+]c with membrane depolarization but the increase in membrane conductance started later than the other two processes. There was a close temporal relationship between the increase in [Ca2+]c and membrane depolarization on the initial response of turgor regulation induced by hypotonic treatment.

3 Corresponding author: E-mail, bio004@art.osaka-med.ac.jp; Fax: +81-726-84-7049.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Plant Cell PhysiolHome page
T. Hayashi and S. Takagi
Ca2+-Dependent Cessation of Cytoplasmic Streaming Induced by Hypertonic Treatment in Vallisneria Mesophyll Cells: Possible Role of Cell Wall-Plasma Membrane Adhesion
Plant Cell Physiol., October 15, 2003; 44(10): 1027 - 1036.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.